The use of a double antibody sandwich ELISA and monoclonal antibodies for the assessment of porcine IgM, IgG and IgA concentrations

Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) have been developed for the assessment of IgM, IgG and IgA concentrations in porcine serum. Isotype-specific monoclonal antibodies (mAb) were used for coating and detection. The DAS-ELISAs were examined for their ability to dete...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1995-02, Vol.44 (3), p.309-317
Main Authors: Bianchi, A.T.J., Moonen-Leusen, H.W.M., van der Heijden, P.J., Bokhout, B.A.
Format: Article
Language:English
Subjects:
Aging - immunology
Animals
Antibodies, Monoclonal - immunology
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
CERDO
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Immunoglobulin A - analysis
Immunoglobulin G - analysis
Immunoglobulin M - analysis
IMMUNOGLOBULINE
IMMUNOGLOBULINS
INMUNOGLOBULINA
MONOCLONAL ANTIBODIES
PORCIN
Reproducibility of Results
Sensitivity and Specificity
SWINE
Swine - immunology
TEST ELISA
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Summary:Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) have been developed for the assessment of IgM, IgG and IgA concentrations in porcine serum. Isotype-specific monoclonal antibodies (mAb) were used for coating and detection. The DAS-ELISAs were examined for their ability to detect each isotype and for the assay variations. A computer programme was used to verify the parallelism of the slope of each serum sample with the slope of standard/reference serum, a prerequisite for reliable estimation of Ig concentrations. The DAS-ELISAs are easy to perform and highly specific, have adequate detection levels (ranging from 7 to 50 ng ml −1) and are very reproducible, as illustrated by the inter- and intra-assay variation coefficients (ranging between 4.9 and 7%). To illustrate the applicability of the ELISAs we assessed Ig concentrations in pig sera sampled from birth to young adulthood.
ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(94)05307-E