Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells

A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phospho...

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Bibliographic Details
Published in:FEBS letters 1995-05, Vol.364 (2), p.223-228
Main Authors: Doza, Yair N., Cuenda, Ana, Thomas, Gareth M., Cohen, Philip, Nebreda, Angel R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Arsenite
Binding Sites
Cell Line
DNA, Complementary - genetics
Enzyme Activation
Humans
Intracellular Signaling Peptides and Proteins
MAP kinase
Molecular Sequence Data
Mutation
Peptide Fragments - chemistry
Peptide Fragments - genetics
Phosphorylation
Phosphotyrosine
Protein kinase
Protein Kinases
Protein-Serine-Threonine Kinases - chemistry
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - chemistry
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - metabolism
Stress
Xenopus
Xenopus Proteins
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Summary:A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00346-B