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Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61)
This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Bio...
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| Published in: | Veterinary immunology and immunopathology 1995-02, Vol.44 (3-4), p.359-368 |
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| Language: | English |
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| container_end_page | 368 |
| container_issue | 3-4 |
| container_start_page | 359 |
| container_title | Veterinary immunology and immunopathology |
| container_volume | 44 |
| creator | Pintado, C.O. (Cordoba Univ. (Spain). Facultad de Veterinaria, Dept. de Genetica) Friend, M Llanes, D |
| description | This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments. |
| doi_str_mv | 10.1016/0165-2427(94)05310-O |
| format | article |
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(Cordoba Univ. (Spain). Facultad de Veterinaria, Dept. de Genetica) ; Friend, M ; Llanes, D</creator><creatorcontrib>Pintado, C.O. (Cordoba Univ. (Spain). Facultad de Veterinaria, Dept. de Genetica) ; Friend, M ; Llanes, D</creatorcontrib><description>This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/0165-2427(94)05310-O</identifier><identifier>PMID: 7538249</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; Antigens, CD - analysis ; Blood Platelets - chemistry ; CABALLOS ; CHEVAL ; Epitopes ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; HORSES ; Horses - blood ; LEUCOCITOS ; LEUCOCYTE ; LEUKOCYTES ; Leukocytes - chemistry ; Mice ; MONOCLONAL ANTIBODIES ; Platelet Membrane Glycoproteins - analysis ; Platelet Membrane Glycoproteins - immunology ; Receptors, Cell Surface - analysis ; RUMIANTE ; RUMINANT ; RUMINANTS ; Ruminants - blood ; Species Specificity</subject><ispartof>Veterinary immunology and immunopathology, 1995-02, Vol.44 (3-4), p.359-368</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7538249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pintado, C.O. (Cordoba Univ. (Spain). Facultad de Veterinaria, Dept. de Genetica)</creatorcontrib><creatorcontrib>Friend, M</creatorcontrib><creatorcontrib>Llanes, D</creatorcontrib><title>Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61)</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>Antigens, CD - analysis</subject><subject>Blood Platelets - chemistry</subject><subject>CABALLOS</subject><subject>CHEVAL</subject><subject>Epitopes</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>HORSES</subject><subject>Horses - blood</subject><subject>LEUCOCITOS</subject><subject>LEUCOCYTE</subject><subject>LEUKOCYTES</subject><subject>Leukocytes - chemistry</subject><subject>Mice</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>Platelet Membrane Glycoproteins - analysis</subject><subject>Platelet Membrane Glycoproteins - immunology</subject><subject>Receptors, Cell Surface - analysis</subject><subject>RUMIANTE</subject><subject>RUMINANT</subject><subject>RUMINANTS</subject><subject>Ruminants - blood</subject><subject>Species Specificity</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EKtvCC6Ai-VS1h7D-k8TxsVpoibSiFzhHE2fSdXHi1HYO-1Y8Ipa6gmMPoxnN99PM6BtCLjn7whmvtzmqQpRCXevyhlWSs-LhDdnwRslCVLJ8Szb_kPfkPMYnxlilm-aMnKlKNqLUG_Jnd4AAJmGwEZL1M_UjBTrh1AeYkQY0uCQfaFbCOtkZ5hQpzAPF59VmYHGQ0OGpueQ5ywEDONo77wfqcP3tzTFhpNFO1kGgydN0QHpYJ5ipnRM-Bjv_X_TojsYvwSfM3bbtt23bAr3efS35tuY3H8i7EVzEj6d8QX7dffu5-17sH-7b3e2-GEVVp6IfjOFMNEyPzAihe1CsryEXstEcRxwHVRkOUmsjWKmQa9ScQz1k_7gu5QW5epmbT3leMaZustGgc9kVv8ZOKaEEV_pVkNfZcl3XGfx8Atd-wqFbgp0gHLvTL7L-6UUfwXeQTYndj72umJT5m38B49uWpA</recordid><startdate>19950201</startdate><enddate>19950201</enddate><creator>Pintado, C.O. 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(Cordoba Univ. (Spain). Facultad de Veterinaria, Dept. de Genetica)</creatorcontrib><creatorcontrib>Friend, M</creatorcontrib><creatorcontrib>Llanes, D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pintado, C.O. (Cordoba Univ. (Spain). 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Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments.</abstract><cop>Netherlands</cop><pmid>7538249</pmid><doi>10.1016/0165-2427(94)05310-O</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 1995-02, Vol.44 (3-4), p.359-368 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Animals Antibodies, Monoclonal - biosynthesis ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Antigens, CD - analysis Blood Platelets - chemistry CABALLOS CHEVAL Epitopes GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS HORSES Horses - blood LEUCOCITOS LEUCOCYTE LEUKOCYTES Leukocytes - chemistry Mice MONOCLONAL ANTIBODIES Platelet Membrane Glycoproteins - analysis Platelet Membrane Glycoproteins - immunology Receptors, Cell Surface - analysis RUMIANTE RUMINANT RUMINANTS Ruminants - blood Species Specificity |
| title | Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61) |
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