Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro- s-triazine (mPEG). Herein...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1995-04, Vol.696 (2), p.219-225
Main Authors: Brumeanu, T.-D., Zaghouani, H., Bona, C.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Chromatography, Gel
Chromatography, Ion Exchange
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Epitopes
Fluorescamine - analysis
Genetic Engineering
Immunoconjugates - immunology
Immunoconjugates - isolation & purification
Immunoglobulins - immunology
Immunoglobulins - isolation & purification
Mice
Polyethylene Glycols - chemistry
Rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro- s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10 −4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)01275-J