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Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro- s-triazine (mPEG). Herein...

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Published in:	Journal of Chromatography A 1995-04, Vol.696 (2), p.219-225
Main Authors:	Brumeanu, T.-D., Zaghouani, H., Bona, C.
Format:	Article
Language:	English
Subjects:	Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Epitopes Fluorescamine - analysis Genetic Engineering Immunoconjugates - immunology Immunoconjugates - isolation & purification Immunoglobulins - immunology Immunoglobulins - isolation & purification Mice Polyethylene Glycols - chemistry Rats
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cited_by	cdi_FETCH-LOGICAL-c357t-ad35b0b2dd8e47b7863e06e91ac695ddb3a379b77bb1a0c44f4800f9015178243
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container_end_page	225
container_issue	2
container_start_page	219
container_title	Journal of Chromatography A
container_volume	696
creator	Brumeanu, T.-D. Zaghouani, H. Bona, C.
description	Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro- s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10 −4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.
doi_str_mv	10.1016/0021-9673(94)01275-J
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source	ScienceDirect Freedom Collection 2022-2024
subjects	Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Epitopes Fluorescamine - analysis Genetic Engineering Immunoconjugates - immunology Immunoconjugates - isolation & purification Immunoglobulins - immunology Immunoglobulins - isolation & purification Mice Polyethylene Glycols - chemistry Rats
title	Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol
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