Effect of n-3 fatty acid ethyl ester supplementation on fatty acid composition of the single platelet phospholipids and on platelet functions

• Published in: Metabolism, clinical and experimental clinical and experimental, 1995-05, Vol.44 (5), p.562-569
• Main Authors: Prisco, Domenico, Filippini, Monica, Francalanci, Isa, Paniccia, Rita, Gensini, Gian Franco, Serneri, Gian Gastone Neri
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Blood Platelets - chemistry; Blood Platelets - metabolism; Blood Platelets - physiology; Blood Pressure; Collagen - pharmacology; Dietary Fats, Unsaturated - pharmacology; Fatty Acids - analysis; Fatty Acids, Omega-3 - pharmacology; General and cellular metabolism. Vitamins; Humans; Male; Medical sciences; Olive Oil; Pharmacology. Drug treatments; Phospholipids - blood; Phospholipids - chemistry; Plant Oils - pharmacology; Platelet Aggregation - drug effects; Reference Values; Thromboxane B2 - blood; Time Factors
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/0026-0495(95)90111-6

Twenty healthy male volunteers were randomly assigned to receive either four 1-g capsules of n-3 polyunsaturated fatty acids (PUFA) ethyl esters or four 1-g capsules of olive oil (as placebo) for a period of 4 months, followed by a 3-month wash-out period. Fatty acids of platelet phospholipid fractions, platelet aggregation, and thromboxane B 2 (TXB 2) formation were analyzed at 0, 2, and 4 months of treatment and at 1, 2, and 3 months of wash-out. During n-3 PUFA supplementation, accumulations of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids were markedly increased after 2 months, with slight differences in further accumulation up to 4 months among the various phospholipid fractions. Significant decreases in platelet sensitivity to collagen, serum TXB 2 levels, and urinary TXB 2 metabolites were also observed following n-3 PUFA treatment. During the first and second month of wash-out, slight differences were observed in changes of various fatty acids among different phospholipid fractions, but after 3 months of wash-out, alterations were no longer detectable with respect to pretreatment values. After 3 months of wash-out, platelet function parameters also were returned to baseline. Thus, both platelet lipids and function are influenced by n-3 PUFA ethyl ester supplementation, and significant alterations are still detectable after 2 months of wash-out.