Induction, collection, and partial characterization of induced respiratory macrophages of the turkey

Respiratory macrophages (RM) of the turkey were elicited with a 1:4 (v/v) suspension of incomplete Freund's adjuvant in sterile phosphate-buffered saline injected directly into the abdominal air sacs. RM were purified by passage through a Ficoll-Hypaque gradient resulting in 95.7 ± 5.9% purity...

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Bibliographic Details
Published in:Avian diseases 1986-10, Vol.30 (4), p.766-771
Main Authors: Ficken, Martin D., Edwards, John F., Lay, John C.
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacteriophages
Chemical suspensions
Chickens
dindon
enfermedades respiratorias
escherichia
fagocitos
Female
immune response
Lung - cytology
Macrophage Activation
Macrophages
Macrophages - cytology
maladie respiratoire
Male
pavo
phagocyte
Phagocytes
Pulmonary alveoli
reponse immunitaire
Respiratory diseases
respuesta inmunologica
Therapeutic irrigation
Turkeys
Turkeys - physiology
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Summary:Respiratory macrophages (RM) of the turkey were elicited with a 1:4 (v/v) suspension of incomplete Freund's adjuvant in sterile phosphate-buffered saline injected directly into the abdominal air sacs. RM were purified by passage through a Ficoll-Hypaque gradient resulting in 95.7 ± 5.9% purity and 94.8 ± 12.3% viability. On days 7 and 9 postinjection, adequate numbers (7.15± 5.47× 106macrophages per turkey) of RM for in vitro experiments were obtained. RM of the turkey demonstrated the ability to adhere to glass, phagocytize Zymosan A, and kill Escherichia coli in vitro. /// Se indujeron macrófagos respiratorios de pavo mediante la inyección de una suspensión 1:4 (v/v) de adyuvante incompleto de Freund en una solución salina fosfatada y buferada estéril, aplicada directamente en los sacos aéreos abdominales. Los macrófagos respiratorios fueron purificados a través de un gradiente de Ficoll-Hypaque, resultando en una pureza de 95.7 ± 5.9% y una viabilidad del 94.8 ± 12.3%. Siete y nueve días después de la infección, se obtuvo un número adecuado de macrófagos respiratorios (7.15± 5.47× 106macrófagos por pavo) para los experimentos in vitro. Estos macrófagos respiratorios demonstraron tener capacidad para adherirse al vidrio, fagocitar el Zymosan A y destruir al Escherichia coli in vitro.
ISSN:0005-2086
1938-4351
DOI:10.2307/1590582