Identification and intracellular location of MAGE-3 gene product

The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein charact...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1995-06, Vol.55 (11), p.2236-2239
Main Authors: KOCHER, T, SCHULTZ-THATER, E, GUDAT, F, SCHAEFER, C, CASORATI, G, JURETIC, A, WILLIMANN, T, HARDER, F, HEBERER, M, SPAGNOLI, G. C
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibody Specificity
Antigens, Neoplasm - analysis
Antigens, Neoplasm - genetics
Antigens, Neoplasm - immunology
Base Sequence
Biological and medical sciences
Cloning, Molecular
Gene Expression
Host-tumor relations. Immunology. Biological markers
Intracellular Fluid - chemistry
Intracellular Fluid - immunology
Medical sciences
Melanoma - chemistry
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neoplasm Proteins
Tumor Cells, Cultured
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. The semipurified product was used to generate MAGE-3-specific monoclonal antibodies. One reagent could identify by immunoblotting the native MAGE-3 gene product as a M(r) 48,000 protein in lysates of cell lines showing evidence of MAGE-3 gene expression. No apparent cross-reactivity with recombinant or native MAGE-1 gene product was observed. Immunohistochemistry shows that, closely resembling the MAGE-1 gene product, MAGE-3 is a cytoplasmic protein.
ISSN:0008-5472
1538-7445