Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster

In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, cor...

Full description

Saved in:
Bibliographic Details
Published in:Gene 1995-04, Vol.156 (2), p.253-257
Main Authors: Di Gregorio, Anna, Spagnuolo, Antonietta, Ristoratore, Filomena, Pischetola, Michele, Aniello, Francesco, Branno, Margherita, Cariello, Lucio, Di Lauro, Roberto
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antennapedia Homeodomain Protein
Biological Evolution
Ciona intestinalis - genetics
development
Distal-less
DNA-Binding Proteins - genetics
Genes, Homeobox - genetics
Genomic Library
HLX
homeodomain
Homeodomain Proteins
Hox
IPF1
Molecular Sequence Data
Multigene Family - genetics
Nuclear Proteins
Oligonucleotide Probes
Ovum - chemistry
paired
RNA, Messenger - analysis
sea squirt
Selection, Genetic
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Space life sciences
Transcription Factors
Tunicate
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, corresponding to several classes of homeodomains, have been identified. Five of the isolated homeoboxes show their highest homology to members of the Vertebrate HOX clusters. mRNAs for two of the isolated homeoboxes are present in unfertilized C. intestinalis eggs
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00035-5