cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA penta...

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Bibliographic Details
Published in:Neuroscience letters 1995-02, Vol.185 (3), p.175-178
Main Authors: Yokoji, Hidehiro, Ariyama, Takeshi, Takahashi, Ryosuke, Inazawa, Johji, Misawa, Hidemi, Deguchi, Takeo
Format: Article
Language:English
Subjects:
Biological and medical sciences
cDNA
Chromosomal localization
Chromosomes - genetics
Ciliary Neurotrophic Factor
Cloning, Molecular
DNA, Complementary
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Gene structure
Genes. Genome
Human ciliary neurotrophic factor
Humans
In Situ Hybridization
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nerve Tissue Proteins - genetics
Sequence Analysis
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Summary:Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(95)11254-T