A Subpopulation of Smooth Muscle Cells in Injured Rat Arteries Expresses Platelet-Derived Growth Factor-B Chain mRNA

Proliferation of smooth muscle cells (SMCs) and formation of a neointima are characteristics of the response of rat carotid arteries to balloon injury.Rat platelet-derived growth factor (PDGF)-B was cloned, thus allowing us to use species-specific probes to carry out in situ hybridization on the sur...

Full description

Saved in:
Bibliographic Details
Published in:Circulation research 1995-06, Vol.76 (6), p.951-957
Main Authors: Lindner, Volkhard, Giachelli, Cecilia M, Schwartz, Stephen M, Reidy, Michael A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Angioplasty, Balloon - adverse effects
Animals
Base Sequence
Biological and medical sciences
Blood vessels and receptors
Carotid Arteries - cytology
Carotid Artery Injuries
Cells, Cultured
Cloning, Molecular
DNA Probes
Fundamental and applied biological sciences. Psychology
Gene Expression
In Situ Hybridization
Male
Molecular Sequence Data
Muscle, Smooth, Vascular - cytology
Platelet-Derived Growth Factor - genetics
Population
Protein-Tyrosine Kinases - genetics
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-sis
Rats
Rats, Sprague-Dawley
Receptors, Platelet-Derived Growth Factor
RNA, Messenger - genetics
Vertebrates: cardiovascular system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proliferation of smooth muscle cells (SMCs) and formation of a neointima are characteristics of the response of rat carotid arteries to balloon injury.Rat platelet-derived growth factor (PDGF)-B was cloned, thus allowing us to use species-specific probes to carry out in situ hybridization on the surface of injured arteries. A distinct population of luminal SMCs (7% to 10%) in the developing neointima expressed PDGF-B mRNA, but very few luminal SMCs still expressed PDGF-B (0.5%) when the lesion had stopped growing. Primary SMC cultures revealed expression of PDGF-B mRNA in 1.6% of SMCs derived from normal tunica media and in 11% of SMCs derived from the neointima. These data demonstrate that SMCs in the injured vessel wall are heterogeneous with regard to PDGF-B expression and that subculturing of these cells may give rise to cultures that are either positive or negative for PDGF-B expression. Furthermore, with abundant expression of the PDGF receptor beta-subunit expressed by intimal SMCs, our findings provide evidence that PDGF-B synthesized by these cells may be involved in intimal lesion formation via a paracrine or autocrine mechanism.(Circ Res. 1995;76:951-957.)
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.76.6.951