Developmental expression of the C1G5F2 antigen in cultured rat oligodendroglial cells

The C1G5F2 antigen is a newly described minor myelin antigen of the central nervous system. Its expression compared with that of some other main myelin protein components (Wolfgram W1 protein, myelin basic proteins (MBP) and proteolipids) was investigated in rat oligodendrocytes derived from 10-day-...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroimmunology 1995-05, Vol.58 (2), p.201-210
Main Authors: Birling, Marie-christine, Miehe, Monique, Nussbaum, Jean-louis
Format: Article
Language:English
Subjects:
Animals
C1G5F2 antigen
Electron microscopy
Immunocytochemistry
Membrane Glycoproteins - metabolism
Membrane Glycoproteins - ultrastructure
Membrane surface protein
Myelin
Myelin Proteins - metabolism
Nerve Tissue Proteins - metabolism
Nerve Tissue Proteins - ultrastructure
Oligodendrocyte subcultures
Oligodendroglia - immunology
Oligodendroglia - ultrastructure
Rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The C1G5F2 antigen is a newly described minor myelin antigen of the central nervous system. Its expression compared with that of some other main myelin protein components (Wolfgram W1 protein, myelin basic proteins (MBP) and proteolipids) was investigated in rat oligodendrocytes derived from 10-day-old primary glial cell cultures and subcultured for several days in a chemically defined medium. It was demonstrated immunocytochemically that this antigen is detected later than the major myelin markers. All cells immunoreactive with the monoclonal antibody C1G5F2 were always labeled either by W1-, MBP- or proteolipid-specific antisera. It was also shown at the electron microscopic level that this antigen is mainly expressed on the surface of the extremities of the fine oligodendroglial processes. All these observations suggest that the C1G5F2 antigen may be a useful marker for a specific step in the oligodendrocyte maturation stage.
ISSN:0165-5728
1872-8421
DOI:10.1016/0165-5728(95)00018-W