Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize

• Published in: Gene 1995-04, Vol.156 (2), p.155-166
• Main Authors: Theiβen, Günter, Strater, Tim, Fischer, Achim, Saedler, Heinz
• Format: Article
• Language: English
• Subjects: AGAMOUS Protein, Arabidopsis; Amino Acid Sequence; amino acid sequences; Base Sequence; Chromosome Mapping; Cloning, Molecular; complementary DNA; corn evolution; developmental control genes; DNA-binding proteins; DNA-Binding Proteins - genetics; duplicate loci; exon-intron structure; exons; genbank/x80206; genbank/x81199; Genes, Plant - genetics; Genome; Genomic clones; introns; MADS Domain Proteins; Molecular Sequence Data; Multigene Family; nucleotide sequences; Phylogeny; Plant Proteins - genetics; Protein Structure, Secondary; recombinant inbred mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; structural genes; transcription factors; Transcription Factors - genetics; zag1 gene; zag2 gene; Zea mays; Zea mays - genetics; zmm1 gene; zmm2 gene
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(95)00020-7

In order to gain a better understanding of the development and evolution of cereal flowers, we have cloned and sequenced two MADS-box genes from maize and the partial cDNA of a third one. One of the genomic clones was identified as ZAG2 (Zea AGAMOUS 2), while the other has a very similar structure and the potential to encode a protein which shares 94% sequence identity with the putative ZAG2 gene product. The cDNA reveals considerable similarity to ZAG1. Phylogenetic evaluation of the sequence information, as well as chromosomal localization, both suggest that we have identified two pairs of AGAMOUS-like MADS-box genes which were created during duplication of chromosomal segments or complete chromosomes