Improved pertussis toxin production by Bordetella pertussis through adjusting the growth medium's ionic composition

Ionic composition and total ionic concentration of the growth medium were important factors in limiting productivities in aerated reactors used for the production of pertussis toxin and other antigens by Bordetella pertussis. Salt concentration has opposing effects on cell growth of wild-type B. per...

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Bibliographic Details
Published in:Journal of biotechnology 1995-05, Vol.39 (3), p.205-219
Main Authors: Frohlich, Bert T., De Bernardez Clark, Eliana R., Siber, George R., Swartz, Randall W.
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Ammonium Chloride - pharmacology
Ammonium Sulfate - pharmacology
Antigenic modulation
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Biotechnology - methods
Bordetella pertussis
Bordetella pertussis - drug effects
Bordetella pertussis - growth & development
Bordetella pertussis - physiology
Continuous culture
Culture Media
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Growth media
Hydrogen-Ion Concentration
Ionic composition
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Osmolar Concentration
Pertussis Toxin
Pertussis toxin production
Salt concentration
Sodium
Sodium - pharmacology
Sodium Chloride - pharmacology
Specific yield
Virulence Factors, Bordetella - biosynthesis
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Summary:Ionic composition and total ionic concentration of the growth medium were important factors in limiting productivities in aerated reactors used for the production of pertussis toxin and other antigens by Bordetella pertussis. Salt concentration has opposing effects on cell growth of wild-type B. pertussis and specific toxin formation. Sodium ion concentrations below 140 mM correlated with a precipitous decline in specific yields of pertussis toxin, an otherwise growth-associated product. High salt concentrations in the medium resulted in lower final cell concentrations but did not affect initial growth rates. A new medium is proposed that allows a 60 to 70% increase in both cell and toxin yields by replacing the sodium chloride in the “cyclodextrin liquid” (CL) medium with additional monosodium glutamate which provides both the sodium and the carbon and energy source.
ISSN:0168-1656
1873-4863
DOI:10.1016/0168-1656(95)00013-G