Expression of inhibin α- and βA-subunit mRNA and protein in the fetal sheep ovary throughout gestation

• Published in: Molecular and cellular endocrinology 1995-02, Vol.107 (2), p.141-147
• Main Authors: Engelhardt, H., Harkness, L.M., Thomas, G.B., Brooks, A.N., McNeilly, A.S., Baird, D.T.
• Format: Article
• Language: English
• Subjects: Animals; Female; Fetus; Fetus - metabolism; Follicle; Gene Expression Regulation, Developmental; Gestational Age; Immunohistochemistry; In Situ Hybridization; Inhibin; Inhibins - chemistry; Inhibins - genetics; Inhibins - metabolism; mRNA; Ovary; Ovary - embryology; Ovary - metabolism; Pregnancy; Protein Conformation; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sheep
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/0303-7207(94)03435-V

In adult sheep, inhibin expression in developing follicles appears to be associated with antrum formation. Our objective was to investigate using in situ hybridization and immunohistochemistry whether antral follicles present before birth in the sheep expressed mRNA or peptide for inhibin α- and β A-subunits. At days 70 and 100 when only primordial and primary follicles were present, there was no detectable mRNA or peptide for either inhibin subunit. By days 130 and 140 (term = 145 days), many secondary follicles were present, a proportion of which (∼50%) expressed detectable levels of α-subunit mRNA but not peptide. A number of antral follicles were present by this stage, all of which expressed α-subunit mRNA and peptide. Expression of β A-subunit mRNA and peptide was undetectable at all stages of gestation. Our results indicate that even in non-ovulatory follicles present before birth, expression of inhibin, at least the α-subunit, is developmentally linked with antrum formation.