Protection of guinea-pigs from experimental Rocky Mountain spotted fever by immunization with baculovirus-expressed Rickettsia rickettsii rOmpA protein

Baculovirus recombinants that express the Rickettsia rickettsii rOmpA protein were constructed. Monoclonal antibodies (mAbs) against the rOmpA protein reacted with recombinant-infected Spodoptera frugiperda (Sf9) cells in indirect immunofluorescence assays. Sodium dodecyl sulfate-polyacrylamide gel...

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Bibliographic Details
Published in:Vaccine 1995, Vol.13 (1), p.29-35
Main Authors: Sumner, John W., Sims, Kim G., Jones, Dana C., Anderson, Burt E.
Format: Article
Language:English
Subjects:
Animals
Bacterial Outer Membrane Proteins - biosynthesis
Bacterial Outer Membrane Proteins - genetics
Bacterial Vaccines - therapeutic use
Bacteriology
Baculoviridae - genetics
baculovirus
baculovirus expression system
Base Sequence
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Guinea Pigs
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Male
Microbiology
Molecular Sequence Data
Production of active biomolecules
Recombinant Proteins - biosynthesis
Rickettsia rickettsii
Rickettsia rickettsii - immunology
Rocky Mountain Spotted Fever - etiology
Rocky Mountain Spotted Fever - prevention & control
rOmpA protein
Spodoptera - metabolism
Spodoptera frugiperda
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccins
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Summary:Baculovirus recombinants that express the Rickettsia rickettsii rOmpA protein were constructed. Monoclonal antibodies (mAbs) against the rOmpA protein reacted with recombinant-infected Spodoptera frugiperda (Sf9) cells in indirect immunofluorescence assays. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting of infected Sf9 cell lysates with a mAb against rOmpA showed that the recombinant-expressed rOmpA protein migrated slightly below rOmpA extracted from R. rickettssi. Guinea-pigs immunized with lysates of recombinant-infected Sf9 cells developed antibodies reactive with R. rickettsii and were protected against challenge, indicating that the baculovirus-expressed rOmpA protein could be useful in subunit vaccines and for studies of the immune response to R. rickettsii infection.
ISSN:0264-410X
1873-2518
DOI:10.1016/0264-410X(95)80007-Z