Single-Strand Conformation Polymorphism Study of Human Immunodeficiency Virus Type 1 RNA and DNA in Plasma, Peripheral Blood Mononuclear Cells, and Their Virologic Cultures

• Published in: The Journal of infectious diseases 1995-06, Vol.171 (6), p.1619-1622
• Main Authors: Lin, Hsiang Ju, Siwak, Edward B., Lauder, Ian J., Hollinger, F. Blaine
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Base Sequence; Biological and medical sciences; Blood; Blood plasma; Coculture techniques; Concise Communications; DNA; DNA Primers - chemistry; DNA, Viral - genetics; Genes, env; Genes, gag; HIV 1; HIV Seropositivity - microbiology; HIV-1 - genetics; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunopathology; Leukocytes, Mononuclear - microbiology; Medical sciences; Molecular Sequence Data; Nucleic acids; Polymerase chain reaction; Polymorphism, Single-Stranded Conformational; Proviruses; RNA; RNA, Viral - genetics; Virology
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/171.6.1619

Single-strand conformation polymorphism (SSCP) analysis was applied to human immunodeficiency virus type 1 (HIV-1) nucleic acids in plasma and peripheral blood mononuclear cells (PBMC) from 16 patients and to 15 PBMC cocultures and 6 plasma cultures prepared from the specimens. Two hypervariable regions were analyzed: in the gag gene and part of the V3 loop. Random paired matching of SSCP patterns between HIV-1 RNA and provirus DNA was tested, from plasma and PBMC from the same blood specimen, supernatant and PBMC from the same PBMC coculture, supernatant and PBMC from the same plasma culture, provirus DNA in cocultured PBMC and the PBMC inoculum, and HIV-1 RNA in a plasma culture supernatant and in the plasma inoculum. Paired matching was nonrandom for both regions in the first three situations and for gag in the fourth, with P ⩽ .01; matching was random for gag in the last situation. The HIV-1 env target region produced in culture diverged from that in the inoculum in 18 of 21 instances.