Analysis of the Population Structure of Mycobacterium tuberculosis in Ethiopia, Tunisia, and the Netherlands: Usefulness of DNA Typing for Global Tuberculosis Epidemiology

The genetic heterogeneity among Mycobacterium tuberculosis isolates from 501 patients in Ethiopia, Tunisia, and the Netherlands was compared by analysis of DNA polymorphism driven by insertion element IS6110. The percentage of isolates displaying two or more identical patterns differed greatly in th...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1995-06, Vol.171 (6), p.1504-1513
Main Authors: Hermans, Peter W. M., Messadi, Feriele, Guebrexabher, Haimanot, van Soolingen, Dick, de Haas, Petra E. W., Heersma, Herre, de Neeling, Han, Ayoub, Abdelkader, Portaels, Françoise, Frommel, Dominique, Zribi, Mohamed, van Embden, Jan D. A.
Format: Article
Language:English
Subjects:
Bacterial diseases
Biological and medical sciences
Cluster Analysis
Disease transmission
DNA
DNA fingerprinting
DNA, Bacterial - genetics
Epidemiology
Ethiopia
Human bacterial diseases
Humans
Infections
Infectious diseases
Major Articles
Medical sciences
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Netherlands
Polymorphism, Restriction Fragment Length
Refugees
Restriction fragment length polymorphism
Transposons
Tropical medicine
Tuberculosis
Tuberculosis - microbiology
Tuberculosis and atypical mycobacterial infections
Tunisia
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genetic heterogeneity among Mycobacterium tuberculosis isolates from 501 patients in Ethiopia, Tunisia, and the Netherlands was compared by analysis of DNA polymorphism driven by insertion element IS6110. The percentage of isolates displaying two or more identical patterns differed greatly in the three countries: It was highest among Tunisian isolates and lowest in Dutch isolates. In contrast to isolates from Dutch subjects infected with M. tuberculosis, the majority of strains from Ethiopia and Tunisia were from a few families of genetically highly related strains. Furthermore, little overlap was observed among isolates from the three countries, indicating strict isolation of the bacterial .reservoirs in the countries. A few strains from the Netherlands matched strains from Ethiopia and Tunisia. Those strains were invariably isolated from refugees, immigrants, or persons who visited Ethiopia or Tunisia.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/171.6.1504