Development of a quantal assay in primary shrimp cell culture for yellow head baculovirus (YBV) of penaeid shrimp

A 50% tissue culture infectious dose assay (TCID 50) using primary culture of shrimp lymphoid organ (Oka) cells was developed for the quantitative titration of yellow-head baculovirus (YBV), a newly isolated virus of penaeid shrimp. The assay protocol includes the use of Primaria-grade 96-well tissu...

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Bibliographic Details
Published in:Journal of virological methods 1995-03, Vol.52 (1), p.231-236
Main Authors: Lu, Y., Tapay, L.M., Loh, P.C., Brock, J.A., Gose, R.
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - isolation & purification
Baculoviridae - pathogenicity
Baculoviridae - physiology
Biological and medical sciences
Cells, Cultured
Decapoda (Crustacea) - virology
Epithelial Cells
Epithelium - virology
Fundamental and applied biological sciences. Psychology
Lymphoid Tissue - cytology
Lymphoid Tissue - virology
Marine
Microbiology
Organ Culture Techniques
Penaeus monodon
Penaeus stylirostris
Primary Oka cell culture
TCID 50 assay
Techniques used in virology
Virology
Virus Replication
Yellow-head baculovirus
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Summary:A 50% tissue culture infectious dose assay (TCID 50) using primary culture of shrimp lymphoid organ (Oka) cells was developed for the quantitative titration of yellow-head baculovirus (YBV), a newly isolated virus of penaeid shrimp. The assay protocol includes the use of Primaria-grade 96-well tissue culture plates to grow the primary lymphoid organ cells of penaeid shrimp. A 15% gill suspension from YBV-infected shrimp was determined to have an infectious virus titer of 5 · 10 5.75 TCID 50/ml. This report represents the first convenient assay protocol using cell culture derived from penaeid shrimp to titer a shrimp virus.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(94)00131-Y