Improved method of determining the susceptibility of Candida albicans to fluconazole

The MICs of fluconazole for four reference strains of Candida albicans were determined in one laboratory by the macrobroth dilution method recommended by the National Commitee for Clinical Laboratory Standards (NCCLS) and bya modified version of the method which incorporated microbroth disution, RPM...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1995-01, Vol.35 (1), p.155-159
Main Authors: Polanco, Ana M., Rodriguez-Tudel, Juan L., Baquero, Fernando, Sanchez-Sousa, Aurora, Martinez-Suarez, Joaquin V.
Format: Article
Language:English
Subjects:
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Biological and medical sciences
Candida albicans
Candida albicans - drug effects
Fluconazole - pharmacology
Medical sciences
Microbial Sensitivity Tests - methods
Pharmacology. Drug treatments
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Summary:The MICs of fluconazole for four reference strains of Candida albicans were determined in one laboratory by the macrobroth dilution method recommended by the National Commitee for Clinical Laboratory Standards (NCCLS) and bya modified version of the method which incorporated microbroth disution, RPMI 1640 medium supplemented with glucose and the reading of MIC endpoints by spectro-photometry. The results obtained by the two methods were within one two-fold dilution. In addition, the susceptibilities of 58 clinical isolates of C. albicans to fluconazole were determined in two laboratories by the modified NCCLS method. Close interlaboratory agreement was observed, 98.2% of MICs varying by no more than one two-fold dilution.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/35.1.155