Evaluation of sperm motility by laser—Doppler-spectroscopy in an in-vitro fertilization programme

Laser–Doppler-spectroscopy represents a new, fast and reliable technical method for the assessment of several parameters of sperm motility including the percentage of spermatozoa which are motile, and mean spermatozoal velocity. Serial measurements of these parameters in routine andro logical work p...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1986-06, Vol.1 (4), p.233-235
Main Authors: Pusch, H.H., Leitinger, S., Winter, R., Urdl, W.
Format: Article
Language:English
Subjects:
Fertilization in Vitro - instrumentation
Fertilization in Vitro - methods
Humans
Lasers
Male
Sperm Motility
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Summary:Laser–Doppler-spectroscopy represents a new, fast and reliable technical method for the assessment of several parameters of sperm motility including the percentage of spermatozoa which are motile, and mean spermatozoal velocity. Serial measurements of these parameters in routine andro logical work permit standards for four main spermiogram diagnoses to be established. Laser–Doppler-spectroscopy was used to verily alterations in the motility of spermatozoa after capacitatlon in an in-vitro fertilization programme. An enhancement of 16% for total sperm motility and 36% for mean spermatozoal velocity could be observed. The results of in-vitro fertilization after various spermigrain diagnoses, combined with long-term observations of mean spermatozoal velocity, may be considered as another step towards the elucidation of sperm–oocyte interactions. Comparisons between the development of embryos revealed significant differences between normo- and asthenozoospermic men versus oligozoospermic patients.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a136391