A Microtiter Plate Assay Using Cascade Amplification for Detection of Nonisotopically Labeled DNA

We describe a microtiter-plate-based, colorimetric assay for DNA, the enzyme- linked DNA- enzyme- linked coagulation assay (EDNA-ELCA). The EDNA-ELCA uses amplification of the common pathway of coagulation for the ultrasensitive detection of DNA which is tagged by incorporation of functional groups...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1995-02, Vol.225 (1), p.64-72
Main Authors: Rothschild, C.B., Triscott, M.X., Bowden, D.W., Doellgast, G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Base Sequence
Cloning, Molecular
Colorimetry - methods
DNA - chemical synthesis
DNA - chemistry
DNA Primers - chemical synthesis
Fibrinogen
Fluorescein-5-isothiocyanate
Goats - immunology
Indicators and Reagents
Microchemistry - methods
Molecular Sequence Data
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe a microtiter-plate-based, colorimetric assay for DNA, the enzyme- linked DNA- enzyme- linked coagulation assay (EDNA-ELCA). The EDNA-ELCA uses amplification of the common pathway of coagulation for the ultrasensitive detection of DNA which is tagged by incorporation of functional groups such as biotin and fluorescein. The EDNA-ELCA enables detection of attomole amounts of DNA (
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1109