A Microtiter Plate Assay Using Cascade Amplification for Detection of Nonisotopically Labeled DNA

We describe a microtiter-plate-based, colorimetric assay for DNA, the enzyme- linked DNA- enzyme- linked coagulation assay (EDNA-ELCA). The EDNA-ELCA uses amplification of the common pathway of coagulation for the ultrasensitive detection of DNA which is tagged by incorporation of functional groups...

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Published in:Analytical biochemistry 1995-02, Vol.225 (1), p.64-72
Main Authors: Rothschild, C.B., Triscott, M.X., Bowden, D.W., Doellgast, G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Base Sequence
Cloning, Molecular
Colorimetry - methods
DNA - chemical synthesis
DNA - chemistry
DNA Primers - chemical synthesis
Fibrinogen
Fluorescein-5-isothiocyanate
Goats - immunology
Indicators and Reagents
Microchemistry - methods
Molecular Sequence Data
Polymerase Chain Reaction - methods
Sensitivity and Specificity
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cited_by cdi_FETCH-LOGICAL-c379t-ab31cba57c5ce099708f8dac714ae8661b4e8c3b2f7f97f8e3d4ab093936886a3
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container_issue 1
container_start_page 64
container_title Analytical biochemistry
container_volume 225
creator Rothschild, C.B.
Triscott, M.X.
Bowden, D.W.
Doellgast, G.
description We describe a microtiter-plate-based, colorimetric assay for DNA, the enzyme- linked DNA- enzyme- linked coagulation assay (EDNA-ELCA). The EDNA-ELCA uses amplification of the common pathway of coagulation for the ultrasensitive detection of DNA which is tagged by incorporation of functional groups such as biotin and fluorescein. The EDNA-ELCA enables detection of attomole amounts of DNA (
doi_str_mv 10.1006/abio.1995.1109
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subjects Animals
Antibodies
Base Sequence
Cloning, Molecular
Colorimetry - methods
DNA - chemical synthesis
DNA - chemistry
DNA Primers - chemical synthesis
Fibrinogen
Fluorescein-5-isothiocyanate
Goats - immunology
Indicators and Reagents
Microchemistry - methods
Molecular Sequence Data
Polymerase Chain Reaction - methods
Sensitivity and Specificity
title A Microtiter Plate Assay Using Cascade Amplification for Detection of Nonisotopically Labeled DNA
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