Sexual compatibility among Trypanosoma brucei isolates from an epidemic area in southeastern Uganda

For the first time, two clones of Trypanosoma brucei from the same epidemic area in southeastern Uganda were successfully crossed. The cotransmission experiments were as close to natural conditions as possible in that two uncloned isolates from wild-caught Glossina fuscipes fuscipes were directly co...

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Bibliographic Details
Published in:Parasitology research (1987) 1995, Vol.81 (3), p.253-257
Main Authors: DEGEN, R, POSPICHAL, H, ENYARU, J, JENNI, L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromosomes
Clone Cells
Crosses, Genetic
DNA, Protozoan
Genes, Protozoan
Human protozoal diseases
Infectious diseases
Insect Vectors
Isocitrate Dehydrogenase - genetics
Isoenzymes - genetics
Karyotyping
Medical sciences
Mice
Mice, Inbred ICR
Parasitic diseases
Pedigree
Protozoal diseases
Recombination, Genetic
Trypanosoma brucei brucei - genetics
Trypanosomiasis
Tsetse Flies - parasitology
Uganda
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Summary:For the first time, two clones of Trypanosoma brucei from the same epidemic area in southeastern Uganda were successfully crossed. The cotransmission experiments were as close to natural conditions as possible in that two uncloned isolates from wild-caught Glossina fuscipes fuscipes were directly cotransmitted in an initial recombination experiment. From the first uncloned progeny population, which revealed a majority of recombinants, two clones with different parental phenotypes [characterized by isocitrate dehydrogenase (ICD) analysis] were isolated. These clones were cotransmitted in a second recombination experiment. Nine clones could be isolated from two different progeny populations of the second experiment, and all showed a recombinant phenotype. These nine clones belonged to three different karyotypes with respect to the large chromosomes (1-3 Mb), which were different from those of either parental karyotype or the sum of both parental karyotypes. The results indicate that genetic recombination might well occur between trypanosome populations transmitted within the same epidemic area.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00937118