p53 Expression and DNA ploidy of cartilage lesions

The aim of this study was to investigate the expression of a tumor suppressor gene (p53) in cartilage lesions of bone and its relationship to their histological grade and DNA ploidy. An immunohistochemical assay for p53 and Feulgen-stained DNA preparations was subjected to computerized image analysi...

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Bibliographic Details
Published in:Human pathology 1995-06, Vol.26 (6), p.620-624
Main Authors: Coughlan, Brendan, Feliz, Aaron, Ishida, Tsuyoshi, Czerniak, Bogdan, Dorfman, Howard D
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
cartilage lesions
Chondroma - chemistry
Chondroma - genetics
Chondromatosis, Synovial - genetics
Chondrosarcoma - chemistry
Chondrosarcoma - genetics
Diseases of the osteoarticular system
DNA ploidy
DNA, Neoplasm - analysis
Female
Gene Expression Regulation, Neoplastic
Genes, p53
Humans
Male
Medical sciences
Middle Aged
p53 expression
Ploidies
Tumor Suppressor Protein p53 - analysis
Tumor Suppressor Protein p53 - genetics
Tumors of striated muscle and skeleton
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Summary:The aim of this study was to investigate the expression of a tumor suppressor gene (p53) in cartilage lesions of bone and its relationship to their histological grade and DNA ploidy. An immunohistochemical assay for p53 and Feulgen-stained DNA preparations was subjected to computerized image analysis. Enchondromas, synovial chondromatosis, and low grade (grade I and II) chondrosarcomas were diploid. High grade (grade III) chondrosarcomas and high grade sarcomatous components of dedifferentiated chondrosarcomas were aneuploid. Well differentiated cartilaginous components of dedifferentiated chondrosarcomas were diploid. Microscopic examination showed weak focal positivity for p53 in one of 10 enchondromas one of six examples of synovial chondromatosis, and three of four low grade (grade I and II) chondrosarcomas. All three high grade (grade III) chondrosarcomas were strongly positive for p53. The high grade sarcomatous component of all four dedifferentiated chondrosarcomas was strongly positive for p53, whereas only focal weak positivity was noted in the well differentiated cartilaginous areas. These results were confirmed by quantitative computer-assisted image analysis, which showed that high grade aneuploid cartilage tumors demonstrated strikingly higher levels of p53 than did diploid low grade malignant tumors or benign cartilage lesions.
ISSN:0046-8177
1532-8392
DOI:10.1016/0046-8177(95)90166-3