Differential Induction of mRNA for ICAM-1 and Selectins in Hepatocytes, Kupffer Cells and Endothelial Cells During Endotoxemia

Intercellular adhesion molecule-1 (ICAM-1) and selectins (E- and P-selectin) mRNAs were determined in individual liver cell types by Northern blot analysis before and after injection of endotoxin. A constitutive expression of ICAM-1 mRNA was found in endothelial cells and Kupffer cells but not in he...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-06, Vol.211 (1), p.74-82
Main Authors: Essani, N.A., Mcguire, G.M., Manning, A.M., Jaeschke, H.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Base Sequence
Blotting, Northern
Cell Adhesion Molecules - biosynthesis
DNA Probes
E-Selectin
Endothelium - immunology
Endothelium - metabolism
Gene Expression
Intercellular Adhesion Molecule-1 - biosynthesis
Kupffer Cells - immunology
Kupffer Cells - metabolism
Liver - immunology
Liver - metabolism
Male
Molecular Sequence Data
Oligonucleotide Probes
P-Selectin
Platelet Membrane Glycoproteins - biosynthesis
Rats
Rats, Inbred F344
RNA, Messenger - biosynthesis
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Summary:Intercellular adhesion molecule-1 (ICAM-1) and selectins (E- and P-selectin) mRNAs were determined in individual liver cell types by Northern blot analysis before and after injection of endotoxin. A constitutive expression of ICAM-1 mRNA was found in endothelial cells and Kupffer cells but not in hepatocytes. All three cell types showed upregulation of ICAM-1 mRNA after endotoxin. No constitutive selectin expression could be detected in any liver cell, but endotoxin induced massive synthesis of E- and P-selectin mRNA in endothelial cells and Kupffer cells. The differential expression of cellular adhesion molecules in the liver is consistent with the involvement of selectins in neutrophil rolling in the vasculature and ICAM-1 in transendothelial migration and adherence to parenchymal cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1780