Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells

Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunob...

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Bibliographic Details
Published in:Neuroscience letters 1995-02, Vol.186 (2), p.208-210
Main Authors: Oho, Chikara, Seino, Susumu, Takahashi, Masami
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Carrier Proteins - biosynthesis
Cell Membrane - metabolism
Cell physiology
Cells, Cultured
Clone Cells - metabolism
Endocrine cell
Endocrine Glands - cytology
Endocrine Glands - metabolism
Fundamental and applied biological sciences. Psychology
Immunoblotting
Membrane Proteins - biosynthesis
Membrane Proteins - metabolism
Molecular and cellular biology
Molecular Sequence Data
N-Ethyl-maleimide-sensitive factor attachment protein (SNAP) receptor
N-Ethylmaleimide-Sensitive Proteins
Nerve Tissue Proteins - metabolism
Neurotransmitter release
Qa-SNARE Proteins
R-SNARE Proteins
Rats
Secretion. Exocytosis
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Synaptic Membranes - metabolism
Synaptobrevin
Synaptosome-associated protein 25 (SNAP-25)
Syntaxin
Vesicular Transport Proteins
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Summary:Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunoblotting in clonal endocrine cells; catecholamine-secreting adrenal medullary cells (PC12), growth hormone-secreting pituitary cells (GH3), and insulin-secreting pancreatic β-cells (RINr and RINm5F). All of these receptors as well as NSF and SNAP are expressed in these cells and, co-immunoprecipitation experiments indicate that syntaxin is associated with SNAP-25 and synaptobrevin after solubilization. These results suggest that syntaxin, SNAP-25, and synaptobrevin form a complex and are involved in the secretion of hormones from endocrine cells by exocytosis.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(95)11317-P