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Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells
Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunob...
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| Published in: | Neuroscience letters 1995-02, Vol.186 (2), p.208-210 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c483t-347e746c6b6b15e35cd335c92ed89d68046553046cc22e0c35edbd71aabc9ea53 |
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| cites | cdi_FETCH-LOGICAL-c483t-347e746c6b6b15e35cd335c92ed89d68046553046cc22e0c35edbd71aabc9ea53 |
| container_end_page | 210 |
| container_issue | 2 |
| container_start_page | 208 |
| container_title | Neuroscience letters |
| container_volume | 186 |
| creator | Oho, Chikara Seino, Susumu Takahashi, Masami |
| description | Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of
N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunoblotting in clonal endocrine cells; catecholamine-secreting adrenal medullary cells (PC12), growth hormone-secreting pituitary cells (GH3), and insulin-secreting pancreatic β-cells (RINr and RINm5F). All of these receptors as well as NSF and SNAP are expressed in these cells and, co-immunoprecipitation experiments indicate that syntaxin is associated with SNAP-25 and synaptobrevin after solubilization. These results suggest that syntaxin, SNAP-25, and synaptobrevin form a complex and are involved in the secretion of hormones from endocrine cells by exocytosis. |
| doi_str_mv | 10.1016/0304-3940(95)11317-P |
| format | article |
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N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunoblotting in clonal endocrine cells; catecholamine-secreting adrenal medullary cells (PC12), growth hormone-secreting pituitary cells (GH3), and insulin-secreting pancreatic β-cells (RINr and RINm5F). All of these receptors as well as NSF and SNAP are expressed in these cells and, co-immunoprecipitation experiments indicate that syntaxin is associated with SNAP-25 and synaptobrevin after solubilization. These results suggest that syntaxin, SNAP-25, and synaptobrevin form a complex and are involved in the secretion of hormones from endocrine cells by exocytosis.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(95)11317-P</identifier><identifier>PMID: 7777198</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Carrier Proteins - biosynthesis ; Cell Membrane - metabolism ; Cell physiology ; Cells, Cultured ; Clone Cells - metabolism ; Endocrine cell ; Endocrine Glands - cytology ; Endocrine Glands - metabolism ; Fundamental and applied biological sciences. Psychology ; Immunoblotting ; Membrane Proteins - biosynthesis ; Membrane Proteins - metabolism ; Molecular and cellular biology ; Molecular Sequence Data ; N-Ethyl-maleimide-sensitive factor attachment protein (SNAP) receptor ; N-Ethylmaleimide-Sensitive Proteins ; Nerve Tissue Proteins - metabolism ; Neurotransmitter release ; Qa-SNARE Proteins ; R-SNARE Proteins ; Rats ; Secretion. Exocytosis ; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins ; Synaptic Membranes - metabolism ; Synaptobrevin ; Synaptosome-associated protein 25 (SNAP-25) ; Syntaxin ; Vesicular Transport Proteins</subject><ispartof>Neuroscience letters, 1995-02, Vol.186 (2), p.208-210</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-347e746c6b6b15e35cd335c92ed89d68046553046cc22e0c35edbd71aabc9ea53</citedby><cites>FETCH-LOGICAL-c483t-347e746c6b6b15e35cd335c92ed89d68046553046cc22e0c35edbd71aabc9ea53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3433092$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7777198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oho, Chikara</creatorcontrib><creatorcontrib>Seino, Susumu</creatorcontrib><creatorcontrib>Takahashi, Masami</creatorcontrib><title>Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of
N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunoblotting in clonal endocrine cells; catecholamine-secreting adrenal medullary cells (PC12), growth hormone-secreting pituitary cells (GH3), and insulin-secreting pancreatic β-cells (RINr and RINm5F). All of these receptors as well as NSF and SNAP are expressed in these cells and, co-immunoprecipitation experiments indicate that syntaxin is associated with SNAP-25 and synaptobrevin after solubilization. These results suggest that syntaxin, SNAP-25, and synaptobrevin form a complex and are involved in the secretion of hormones from endocrine cells by exocytosis.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - biosynthesis</subject><subject>Cell Membrane - metabolism</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Clone Cells - metabolism</subject><subject>Endocrine cell</subject><subject>Endocrine Glands - cytology</subject><subject>Endocrine Glands - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoblotting</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>N-Ethyl-maleimide-sensitive factor attachment protein (SNAP) receptor</subject><subject>N-Ethylmaleimide-Sensitive Proteins</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurotransmitter release</subject><subject>Qa-SNARE Proteins</subject><subject>R-SNARE Proteins</subject><subject>Rats</subject><subject>Secretion. Exocytosis</subject><subject>Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins</subject><subject>Synaptic Membranes - metabolism</subject><subject>Synaptobrevin</subject><subject>Synaptosome-associated protein 25 (SNAP-25)</subject><subject>Syntaxin</subject><subject>Vesicular Transport Proteins</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkc9qFTEUxgdR6rX6BgpZiLSL0WSSTCabQin1D5R6QV2HTHKGRjLJmOSW9i18ZDPey11qFgmc8zsfX87XNK8Jfk8w6T9gillLJcNnkp8TQolot0-aDRlE1wopuqfN5og8b17k_BNjzAlnJ82JqIfIYdP8vn5YEuTsYkA6WGTivHh4QFNMsy5rNU4oR78bPaDbFsrdo29n7cHNzkKbIWRX3D2gSZsSE9KlaHM3QyhoSbGAC-js2-3l9hwlMLBUJKNaMz4G7VHSBUGw0SQXABnwPr9snk3aZ3h1eE-bHx-vv199bm--fvpydXnTGjbQ0lImQLDe9GM_Eg6UG0vrJTuwg7T9gFnPef19b0zXATaUgx2tIFqPRoLm9LR5t9etNn_tIBc1u7w60AHiLishaMdE1_0XJP0gGeOrItuDJsWcE0xqSW7W6VERrNbE1BqHWuNQkqu_ialtHXtz0N-NM9jj0CGi2n976OtstJ-SDsblI0YZpViuNi_2GNSl3TtIKhsHwYB1dfNF2ej-7eMP9Iu0hA</recordid><startdate>19950217</startdate><enddate>19950217</enddate><creator>Oho, Chikara</creator><creator>Seino, Susumu</creator><creator>Takahashi, Masami</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19950217</creationdate><title>Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells</title><author>Oho, Chikara ; Seino, Susumu ; Takahashi, Masami</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-347e746c6b6b15e35cd335c92ed89d68046553046cc22e0c35edbd71aabc9ea53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Cell Membrane - metabolism</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Clone Cells - metabolism</topic><topic>Endocrine cell</topic><topic>Endocrine Glands - cytology</topic><topic>Endocrine Glands - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoblotting</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>N-Ethyl-maleimide-sensitive factor attachment protein (SNAP) receptor</topic><topic>N-Ethylmaleimide-Sensitive Proteins</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurotransmitter release</topic><topic>Qa-SNARE Proteins</topic><topic>R-SNARE Proteins</topic><topic>Rats</topic><topic>Secretion. Exocytosis</topic><topic>Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins</topic><topic>Synaptic Membranes - metabolism</topic><topic>Synaptobrevin</topic><topic>Synaptosome-associated protein 25 (SNAP-25)</topic><topic>Syntaxin</topic><topic>Vesicular Transport Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oho, Chikara</creatorcontrib><creatorcontrib>Seino, Susumu</creatorcontrib><creatorcontrib>Takahashi, Masami</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oho, Chikara</au><au>Seino, Susumu</au><au>Takahashi, Masami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1995-02-17</date><risdate>1995</risdate><volume>186</volume><issue>2</issue><spage>208</spage><epage>210</epage><pages>208-210</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Syntaxin, synaptosome-associated protein 25 (SNAP-25) and synaptobrevin are neuronal receptors of
N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein (SNAP), cytosolic proteins essential for membrane transport. The expression of these SNAP receptors was investigated by immunoblotting in clonal endocrine cells; catecholamine-secreting adrenal medullary cells (PC12), growth hormone-secreting pituitary cells (GH3), and insulin-secreting pancreatic β-cells (RINr and RINm5F). All of these receptors as well as NSF and SNAP are expressed in these cells and, co-immunoprecipitation experiments indicate that syntaxin is associated with SNAP-25 and synaptobrevin after solubilization. These results suggest that syntaxin, SNAP-25, and synaptobrevin form a complex and are involved in the secretion of hormones from endocrine cells by exocytosis.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7777198</pmid><doi>10.1016/0304-3940(95)11317-P</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1995-02, Vol.186 (2), p.208-210 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | Elsevier |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Carrier Proteins - biosynthesis Cell Membrane - metabolism Cell physiology Cells, Cultured Clone Cells - metabolism Endocrine cell Endocrine Glands - cytology Endocrine Glands - metabolism Fundamental and applied biological sciences. Psychology Immunoblotting Membrane Proteins - biosynthesis Membrane Proteins - metabolism Molecular and cellular biology Molecular Sequence Data N-Ethyl-maleimide-sensitive factor attachment protein (SNAP) receptor N-Ethylmaleimide-Sensitive Proteins Nerve Tissue Proteins - metabolism Neurotransmitter release Qa-SNARE Proteins R-SNARE Proteins Rats Secretion. Exocytosis Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Synaptic Membranes - metabolism Synaptobrevin Synaptosome-associated protein 25 (SNAP-25) Syntaxin Vesicular Transport Proteins |
| title | Expression and complex formation of soluble N-ethyl-maleimide-sensitive factor attachment protein (SNAP) receptors in clonal rat endocrine cells |
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