Loading…
5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide
In the guinea-pig proximal colon, 5-hydroxytryptamine (5-HT) relaxes the longitudinal muscle by stimulating neuronal 5-HT receptors, which induces the release of nitric oxide (NO). It was investigated whether the inhibitory neurotransmitters adenosine 5'-triphosphate (ATP) and/or vasoactive int...
Saved in:
Published in: | Naunyn-Schmiedeberg's archives of pharmacology 1995-02, Vol.351 (2), p.126-135 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c282t-348d9c5003da9973cb0f824d5f266a45ceb77b2850874edc06f0d25c0df72c2a3 |
---|---|
cites | |
container_end_page | 135 |
container_issue | 2 |
container_start_page | 126 |
container_title | Naunyn-Schmiedeberg's archives of pharmacology |
container_volume | 351 |
creator | Briejer, M R Akkermans, L M Meulemans, A L Lefebvre, R A Schuurkes, J A |
description | In the guinea-pig proximal colon, 5-hydroxytryptamine (5-HT) relaxes the longitudinal muscle by stimulating neuronal 5-HT receptors, which induces the release of nitric oxide (NO). It was investigated whether the inhibitory neurotransmitters adenosine 5'-triphosphate (ATP) and/or vasoactive intestinal polypeptide (VIP) could be involved as well. Antagonists to block the contractile response to 5-HT via 5-HT2, 5-HT3 or 5-HT4 receptors were present throughout the experiments and methacholine was administered to precontract the strips. ATP, VIP and 5-HT induced concentration-dependent relaxations, in the case of 5-HT yielding a non-monophasic concentration-response curve. Tetrodotoxin (TTX; 300 nM), NG-nitro-L-arginine (L-NNA, 100 microM) and their combination did not inhibit the relaxations induced by VIP (up to 0.3 microM) or 0.3-3 microM ATP but reduced those by 10 microM ATP. Suramin (300 microM) strongly inhibited the relaxations to ATP and VIP. L-NNA and suramin also inhibited the relaxations to 5-HT. In the presence of L-NNA (100 microM), suramin did not significantly inhibit the relaxations to 5-HT. Suramin did not affect the relaxations to isoprenaline, nitroglycerin or exogenous NO (1 microM), demonstrating its specificity. Apamin (30 nM) inhibited both the relaxations to ATP (by 70-100%) and to 5-HT; relaxations to isoprenaline were partially inhibited, indicating a non-specific component in the inhibitory action of apamin. However, relaxations to exogenous VIP (up to 0.3 microM), NO (1 microM) and to nitroglycerin were not inhibited. In the presence of L-NNA (100 microM), apamin inhibited the relaxations to 5-HT only at 30 microM. alpha, beta-methylene-ATP (alpha, beta-Me-ATP; 100 microM) did not desensitize the responses to ATP. Reactive blue 2 affected the relaxations to isoprenaline at concentrations necessary to significantly inhibit the relaxations to ATP (i.e. from 10 microM onwards). Thus, it was not possible to test either alpha, beta-Me-ATP or reactive blue 2 against the relaxations to 5-HT. alpha-Chymotrypsin (0.015 mg.ml-1) and trypsin (0.005 mg.ml-1) almost abolished the relaxations to VIP, but did not affect those to isoprenaline and 5-HT. The VIP receptor antagonists [p-Cl-D-Phe6, Leu17]VIP (1 microM) and VIP10-28 (1 and 3 microM) did not affect the concentration-response curve to VIP and were hence not tested against 5-HT. Phosphoramidon (1 microM) had no effect on the relaxations to VIP or 5-HT. |
doi_str_mv | 10.1007/BF00169326 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77325613</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77325613</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-348d9c5003da9973cb0f824d5f266a45ceb77b2850874edc06f0d25c0df72c2a3</originalsourceid><addsrcrecordid>eNpFkDFPwzAQhS0EKqWwsCN5YkAKnO04TtigohSpEgyFNXJtpxildrETVP4DPxpTKphOp_veu7uH0CmBSwIgrm4nAKSoGC320JDkjGakInQfDQFomRFalYfoKMY3ACgI5wM0EEIAVHyIvng2nWfW6V4ZjZ3pg18aZxUOppUb2VnvIvYN7l4NXvbWGZmt7RKvg9_YlWyx8q1319i6DxM7u9wKUtf5rSL41vyob-ZPWDqNXx6e0hBLre0WTJizXUjrkp02x-igkW00J7s6Qs-Tu_l4ms0e7x_GN7NM0ZJ2GctLXSkOwLSsKsHUApqS5po3tChkzpVZCLGgJYdS5EYrKBrQlCvQjaCKSjZC57--6Y33Ph1er2xUpm2lM76PtRCM8oKwBF78gir4GINp6nVIb4fPmkD9E339H32Cz3au_WJl9B-6y5p9A_4XfuQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77325613</pqid></control><display><type>article</type><title>5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide</title><source>Springer LINK Archives</source><creator>Briejer, M R ; Akkermans, L M ; Meulemans, A L ; Lefebvre, R A ; Schuurkes, J A</creator><creatorcontrib>Briejer, M R ; Akkermans, L M ; Meulemans, A L ; Lefebvre, R A ; Schuurkes, J A</creatorcontrib><description>In the guinea-pig proximal colon, 5-hydroxytryptamine (5-HT) relaxes the longitudinal muscle by stimulating neuronal 5-HT receptors, which induces the release of nitric oxide (NO). It was investigated whether the inhibitory neurotransmitters adenosine 5'-triphosphate (ATP) and/or vasoactive intestinal polypeptide (VIP) could be involved as well. Antagonists to block the contractile response to 5-HT via 5-HT2, 5-HT3 or 5-HT4 receptors were present throughout the experiments and methacholine was administered to precontract the strips. ATP, VIP and 5-HT induced concentration-dependent relaxations, in the case of 5-HT yielding a non-monophasic concentration-response curve. Tetrodotoxin (TTX; 300 nM), NG-nitro-L-arginine (L-NNA, 100 microM) and their combination did not inhibit the relaxations induced by VIP (up to 0.3 microM) or 0.3-3 microM ATP but reduced those by 10 microM ATP. Suramin (300 microM) strongly inhibited the relaxations to ATP and VIP. L-NNA and suramin also inhibited the relaxations to 5-HT. In the presence of L-NNA (100 microM), suramin did not significantly inhibit the relaxations to 5-HT. Suramin did not affect the relaxations to isoprenaline, nitroglycerin or exogenous NO (1 microM), demonstrating its specificity. Apamin (30 nM) inhibited both the relaxations to ATP (by 70-100%) and to 5-HT; relaxations to isoprenaline were partially inhibited, indicating a non-specific component in the inhibitory action of apamin. However, relaxations to exogenous VIP (up to 0.3 microM), NO (1 microM) and to nitroglycerin were not inhibited. In the presence of L-NNA (100 microM), apamin inhibited the relaxations to 5-HT only at 30 microM. alpha, beta-methylene-ATP (alpha, beta-Me-ATP; 100 microM) did not desensitize the responses to ATP. Reactive blue 2 affected the relaxations to isoprenaline at concentrations necessary to significantly inhibit the relaxations to ATP (i.e. from 10 microM onwards). Thus, it was not possible to test either alpha, beta-Me-ATP or reactive blue 2 against the relaxations to 5-HT. alpha-Chymotrypsin (0.015 mg.ml-1) and trypsin (0.005 mg.ml-1) almost abolished the relaxations to VIP, but did not affect those to isoprenaline and 5-HT. The VIP receptor antagonists [p-Cl-D-Phe6, Leu17]VIP (1 microM) and VIP10-28 (1 and 3 microM) did not affect the concentration-response curve to VIP and were hence not tested against 5-HT. Phosphoramidon (1 microM) had no effect on the relaxations to VIP or 5-HT.</description><identifier>ISSN: 0028-1298</identifier><identifier>EISSN: 1432-1912</identifier><identifier>DOI: 10.1007/BF00169326</identifier><identifier>PMID: 7770095</identifier><language>eng</language><publisher>Germany</publisher><subject>Adenosine Triphosphate - analogs & derivatives ; Adenosine Triphosphate - pharmacology ; Adenosine Triphosphate - physiology ; Animals ; Apamin - pharmacology ; Colon - drug effects ; Colon - innervation ; Female ; Guinea Pigs ; In Vitro Techniques ; Isoproterenol - pharmacology ; Male ; Muscle Relaxation - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - innervation ; Nitric Oxide - physiology ; Nitroglycerin - pharmacology ; Serotonin - pharmacology ; Suramin - pharmacology ; Vasoactive Intestinal Peptide - physiology</subject><ispartof>Naunyn-Schmiedeberg's archives of pharmacology, 1995-02, Vol.351 (2), p.126-135</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-348d9c5003da9973cb0f824d5f266a45ceb77b2850874edc06f0d25c0df72c2a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7770095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Briejer, M R</creatorcontrib><creatorcontrib>Akkermans, L M</creatorcontrib><creatorcontrib>Meulemans, A L</creatorcontrib><creatorcontrib>Lefebvre, R A</creatorcontrib><creatorcontrib>Schuurkes, J A</creatorcontrib><title>5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide</title><title>Naunyn-Schmiedeberg's archives of pharmacology</title><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><description>In the guinea-pig proximal colon, 5-hydroxytryptamine (5-HT) relaxes the longitudinal muscle by stimulating neuronal 5-HT receptors, which induces the release of nitric oxide (NO). It was investigated whether the inhibitory neurotransmitters adenosine 5'-triphosphate (ATP) and/or vasoactive intestinal polypeptide (VIP) could be involved as well. Antagonists to block the contractile response to 5-HT via 5-HT2, 5-HT3 or 5-HT4 receptors were present throughout the experiments and methacholine was administered to precontract the strips. ATP, VIP and 5-HT induced concentration-dependent relaxations, in the case of 5-HT yielding a non-monophasic concentration-response curve. Tetrodotoxin (TTX; 300 nM), NG-nitro-L-arginine (L-NNA, 100 microM) and their combination did not inhibit the relaxations induced by VIP (up to 0.3 microM) or 0.3-3 microM ATP but reduced those by 10 microM ATP. Suramin (300 microM) strongly inhibited the relaxations to ATP and VIP. L-NNA and suramin also inhibited the relaxations to 5-HT. In the presence of L-NNA (100 microM), suramin did not significantly inhibit the relaxations to 5-HT. Suramin did not affect the relaxations to isoprenaline, nitroglycerin or exogenous NO (1 microM), demonstrating its specificity. Apamin (30 nM) inhibited both the relaxations to ATP (by 70-100%) and to 5-HT; relaxations to isoprenaline were partially inhibited, indicating a non-specific component in the inhibitory action of apamin. However, relaxations to exogenous VIP (up to 0.3 microM), NO (1 microM) and to nitroglycerin were not inhibited. In the presence of L-NNA (100 microM), apamin inhibited the relaxations to 5-HT only at 30 microM. alpha, beta-methylene-ATP (alpha, beta-Me-ATP; 100 microM) did not desensitize the responses to ATP. Reactive blue 2 affected the relaxations to isoprenaline at concentrations necessary to significantly inhibit the relaxations to ATP (i.e. from 10 microM onwards). Thus, it was not possible to test either alpha, beta-Me-ATP or reactive blue 2 against the relaxations to 5-HT. alpha-Chymotrypsin (0.015 mg.ml-1) and trypsin (0.005 mg.ml-1) almost abolished the relaxations to VIP, but did not affect those to isoprenaline and 5-HT. The VIP receptor antagonists [p-Cl-D-Phe6, Leu17]VIP (1 microM) and VIP10-28 (1 and 3 microM) did not affect the concentration-response curve to VIP and were hence not tested against 5-HT. Phosphoramidon (1 microM) had no effect on the relaxations to VIP or 5-HT.</description><subject>Adenosine Triphosphate - analogs & derivatives</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Adenosine Triphosphate - physiology</subject><subject>Animals</subject><subject>Apamin - pharmacology</subject><subject>Colon - drug effects</subject><subject>Colon - innervation</subject><subject>Female</subject><subject>Guinea Pigs</subject><subject>In Vitro Techniques</subject><subject>Isoproterenol - pharmacology</subject><subject>Male</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - innervation</subject><subject>Nitric Oxide - physiology</subject><subject>Nitroglycerin - pharmacology</subject><subject>Serotonin - pharmacology</subject><subject>Suramin - pharmacology</subject><subject>Vasoactive Intestinal Peptide - physiology</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNpFkDFPwzAQhS0EKqWwsCN5YkAKnO04TtigohSpEgyFNXJtpxildrETVP4DPxpTKphOp_veu7uH0CmBSwIgrm4nAKSoGC320JDkjGakInQfDQFomRFalYfoKMY3ACgI5wM0EEIAVHyIvng2nWfW6V4ZjZ3pg18aZxUOppUb2VnvIvYN7l4NXvbWGZmt7RKvg9_YlWyx8q1319i6DxM7u9wKUtf5rSL41vyob-ZPWDqNXx6e0hBLre0WTJizXUjrkp02x-igkW00J7s6Qs-Tu_l4ms0e7x_GN7NM0ZJ2GctLXSkOwLSsKsHUApqS5po3tChkzpVZCLGgJYdS5EYrKBrQlCvQjaCKSjZC57--6Y33Ph1er2xUpm2lM76PtRCM8oKwBF78gir4GINp6nVIb4fPmkD9E339H32Cz3au_WJl9B-6y5p9A_4XfuQ</recordid><startdate>199502</startdate><enddate>199502</enddate><creator>Briejer, M R</creator><creator>Akkermans, L M</creator><creator>Meulemans, A L</creator><creator>Lefebvre, R A</creator><creator>Schuurkes, J A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199502</creationdate><title>5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide</title><author>Briejer, M R ; Akkermans, L M ; Meulemans, A L ; Lefebvre, R A ; Schuurkes, J A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-348d9c5003da9973cb0f824d5f266a45ceb77b2850874edc06f0d25c0df72c2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adenosine Triphosphate - analogs & derivatives</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Adenosine Triphosphate - physiology</topic><topic>Animals</topic><topic>Apamin - pharmacology</topic><topic>Colon - drug effects</topic><topic>Colon - innervation</topic><topic>Female</topic><topic>Guinea Pigs</topic><topic>In Vitro Techniques</topic><topic>Isoproterenol - pharmacology</topic><topic>Male</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - innervation</topic><topic>Nitric Oxide - physiology</topic><topic>Nitroglycerin - pharmacology</topic><topic>Serotonin - pharmacology</topic><topic>Suramin - pharmacology</topic><topic>Vasoactive Intestinal Peptide - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Briejer, M R</creatorcontrib><creatorcontrib>Akkermans, L M</creatorcontrib><creatorcontrib>Meulemans, A L</creatorcontrib><creatorcontrib>Lefebvre, R A</creatorcontrib><creatorcontrib>Schuurkes, J A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Briejer, M R</au><au>Akkermans, L M</au><au>Meulemans, A L</au><au>Lefebvre, R A</au><au>Schuurkes, J A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide</atitle><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><date>1995-02</date><risdate>1995</risdate><volume>351</volume><issue>2</issue><spage>126</spage><epage>135</epage><pages>126-135</pages><issn>0028-1298</issn><eissn>1432-1912</eissn><abstract>In the guinea-pig proximal colon, 5-hydroxytryptamine (5-HT) relaxes the longitudinal muscle by stimulating neuronal 5-HT receptors, which induces the release of nitric oxide (NO). It was investigated whether the inhibitory neurotransmitters adenosine 5'-triphosphate (ATP) and/or vasoactive intestinal polypeptide (VIP) could be involved as well. Antagonists to block the contractile response to 5-HT via 5-HT2, 5-HT3 or 5-HT4 receptors were present throughout the experiments and methacholine was administered to precontract the strips. ATP, VIP and 5-HT induced concentration-dependent relaxations, in the case of 5-HT yielding a non-monophasic concentration-response curve. Tetrodotoxin (TTX; 300 nM), NG-nitro-L-arginine (L-NNA, 100 microM) and their combination did not inhibit the relaxations induced by VIP (up to 0.3 microM) or 0.3-3 microM ATP but reduced those by 10 microM ATP. Suramin (300 microM) strongly inhibited the relaxations to ATP and VIP. L-NNA and suramin also inhibited the relaxations to 5-HT. In the presence of L-NNA (100 microM), suramin did not significantly inhibit the relaxations to 5-HT. Suramin did not affect the relaxations to isoprenaline, nitroglycerin or exogenous NO (1 microM), demonstrating its specificity. Apamin (30 nM) inhibited both the relaxations to ATP (by 70-100%) and to 5-HT; relaxations to isoprenaline were partially inhibited, indicating a non-specific component in the inhibitory action of apamin. However, relaxations to exogenous VIP (up to 0.3 microM), NO (1 microM) and to nitroglycerin were not inhibited. In the presence of L-NNA (100 microM), apamin inhibited the relaxations to 5-HT only at 30 microM. alpha, beta-methylene-ATP (alpha, beta-Me-ATP; 100 microM) did not desensitize the responses to ATP. Reactive blue 2 affected the relaxations to isoprenaline at concentrations necessary to significantly inhibit the relaxations to ATP (i.e. from 10 microM onwards). Thus, it was not possible to test either alpha, beta-Me-ATP or reactive blue 2 against the relaxations to 5-HT. alpha-Chymotrypsin (0.015 mg.ml-1) and trypsin (0.005 mg.ml-1) almost abolished the relaxations to VIP, but did not affect those to isoprenaline and 5-HT. The VIP receptor antagonists [p-Cl-D-Phe6, Leu17]VIP (1 microM) and VIP10-28 (1 and 3 microM) did not affect the concentration-response curve to VIP and were hence not tested against 5-HT. Phosphoramidon (1 microM) had no effect on the relaxations to VIP or 5-HT.</abstract><cop>Germany</cop><pmid>7770095</pmid><doi>10.1007/BF00169326</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0028-1298 |
ispartof | Naunyn-Schmiedeberg's archives of pharmacology, 1995-02, Vol.351 (2), p.126-135 |
issn | 0028-1298 1432-1912 |
language | eng |
recordid | cdi_proquest_miscellaneous_77325613 |
source | Springer LINK Archives |
subjects | Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Adenosine Triphosphate - physiology Animals Apamin - pharmacology Colon - drug effects Colon - innervation Female Guinea Pigs In Vitro Techniques Isoproterenol - pharmacology Male Muscle Relaxation - drug effects Muscle, Smooth - drug effects Muscle, Smooth - innervation Nitric Oxide - physiology Nitroglycerin - pharmacology Serotonin - pharmacology Suramin - pharmacology Vasoactive Intestinal Peptide - physiology |
title | 5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T06%3A27%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=5-HT-induced%20neurogenic%20relaxations%20of%20the%20guinea-pig%20proximal%20colon:%20investigation%20into%20the%20role%20of%20ATP%20and%20VIP%20in%20addition%20to%20nitric%20oxide&rft.jtitle=Naunyn-Schmiedeberg's%20archives%20of%20pharmacology&rft.au=Briejer,%20M%20R&rft.date=1995-02&rft.volume=351&rft.issue=2&rft.spage=126&rft.epage=135&rft.pages=126-135&rft.issn=0028-1298&rft.eissn=1432-1912&rft_id=info:doi/10.1007/BF00169326&rft_dat=%3Cproquest_cross%3E77325613%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c282t-348d9c5003da9973cb0f824d5f266a45ceb77b2850874edc06f0d25c0df72c2a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=77325613&rft_id=info:pmid/7770095&rfr_iscdi=true |