Metal ion binding to apo, holo, and reconstituted horse spleen ferritin

The binding of Cd 2+, Zn 2+, Cu 2+, Ni 2+, Co 2+, Mn 2+, and Mg 2+ to apo, holo, reconstituted horse spleen ferritin (HoSF), and native holo HoSF with phosphate removed was measured by gel-exclusion chromatography. Three classes of strong binding interactions (K d < 10 −7 M) with apo HoSF at pH 7...

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Published in:Journal of inorganic biochemistry 1995-07, Vol.59 (1), p.15-27
Main Authors: Pead, S., Durrant, E., Webb, B., Larsen, C., Heaton, D., Johnson, J., Watt, G.D.
Format: Article
Language:English
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Summary:The binding of Cd 2+, Zn 2+, Cu 2+, Ni 2+, Co 2+, Mn 2+, and Mg 2+ to apo, holo, reconstituted horse spleen ferritin (HoSF), and native holo HoSF with phosphate removed was measured by gel-exclusion chromatography. Three classes of strong binding interactions (K d < 10 −7 M) with apo HoSF at pH 7.5 were found for the various M 2+ studied: high stoichiometric binding (30–54 M 2+/HoSF) for Cd 2+, Zn 2+, Cu 2+, with two protons released per metal bound; intermediate binding (16 M 2+/HoSF) for Ni 2+ and Co 2+, with one proton released per metal bound; and low levels of binding (2–12 M 2+/HoSF) for Mn 2+, Mg 2+, and Fe 2+, with
ISSN:0162-0134
1873-3344
DOI:10.1016/0162-0134(94)00050-K