Metal ion binding to apo, holo, and reconstituted horse spleen ferritin
The binding of Cd 2+, Zn 2+, Cu 2+, Ni 2+, Co 2+, Mn 2+, and Mg 2+ to apo, holo, reconstituted horse spleen ferritin (HoSF), and native holo HoSF with phosphate removed was measured by gel-exclusion chromatography. Three classes of strong binding interactions (K d < 10 −7 M) with apo HoSF at pH 7...
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Published in: | Journal of inorganic biochemistry 1995-07, Vol.59 (1), p.15-27 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The binding of Cd
2+, Zn
2+, Cu
2+, Ni
2+, Co
2+, Mn
2+, and Mg
2+ to apo, holo, reconstituted horse spleen ferritin (HoSF), and native holo HoSF with phosphate removed was measured by gel-exclusion chromatography. Three classes of strong binding interactions (K
d < 10
−7 M) with apo HoSF at pH 7.5 were found for the various M
2+ studied: high stoichiometric binding (30–54 M
2+/HoSF) for Cd
2+, Zn
2+, Cu
2+, with two protons released per metal bound; intermediate binding (16 M
2+/HoSF) for Ni
2+ and Co
2+, with one proton released per metal bound; and low levels of binding (2–12 M
2+/HoSF) for Mn
2+, Mg
2+, and Fe
2+, with |
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ISSN: | 0162-0134 1873-3344 |
DOI: | 10.1016/0162-0134(94)00050-K |