A Quantitative Test for Copper Using Bicinchoninic Acid

We describe a direct colorimetric assay for copper in serum and biological samples using 2,2′-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BC...

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Bibliographic Details
Published in:Analytical biochemistry 1995-03, Vol.226 (1), p.80-84
Main Authors: Brenner, A.J., Harris, E.D.
Format: Article
Language:English
Subjects:
Animals
Cations - pharmacology
Colorimetry - methods
Copper - analysis
Copper - blood
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Quinolines - chemistry
Sensitivity and Specificity
Spectrophotometry
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Description
Summary:We describe a direct colorimetric assay for copper in serum and biological samples using 2,2′-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 × 103 liter mol−1 cm−1 and 4.6 × 104 liter mol−1 cm−1, respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1194