Uptake of pyruvate by early human embryos determined by a non-invasive technique

Normal levels of pyruvate in freshly collected follicular fluid were found to be 0.45 mM, a value ˜4× that of serum. The utilization of pyruvate by human oocytes and early embryos has been measured, non-invasively, using two similar but separate analytical methods. The mature oocyte has a high level...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1986-04, Vol.1 (3), p.181-182
Main Authors: Leese, H.J., Hooper, M.A.K., Edwards, R.G., Ashwood-Smith, M.J.
Format: Article
Language:English
Subjects:
Blastocyst - metabolism
Humans
Morula - metabolism
Oocytes - metabolism
Pyruvates - metabolism
Spectrometry, Fluorescence - methods
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Summary:Normal levels of pyruvate in freshly collected follicular fluid were found to be 0.45 mM, a value ˜4× that of serum. The utilization of pyruvate by human oocytes and early embryos has been measured, non-invasively, using two similar but separate analytical methods. The mature oocyte has a high level of pyruvate uptake (36 pmol/embryo/h). After fertilization this value falls, and then slowly increases to reach a peak just prior to the morula stage of 27 pmol/embryo/h before decreasing. Degenerating oocytes and embryos demonstrated much lower pyruvate uptake rates. The possible usefulness of this method for assessing embryo viability is discussed.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a136376