Structure-activity relationship of the effect of cis-unsaturated fatty acids on heart sarcolemmal phospholipase D activity

This study examined the role of fatty acids on the phosphatidylcholine-specific phospholipase D (PLD) function of purified sarcolemmal (SL) membranes isolated from rat hearts. The enzyme's hydrolytic activity was determined by measuring [ 14C] phosphatidic acid formation from exogenous [ 14C] p...

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Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 1995-02, Vol.52 (2), p.167-171
Main Authors: Dai, J., Williams, S.A., Ziegelhöffer, A., Panagia, V.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fatty Acids - chemistry
Fatty Acids - pharmacology
Fatty Acids, Unsaturated - pharmacology
Fundamental and applied biological sciences. Psychology
Heart
Male
Myocardium - enzymology
Phosphatidylcholines - metabolism
Phospholipase D - metabolism
Rats
Rats, Sprague-Dawley
Sarcolemma - enzymology
Stimulation, Chemical
Structure-Activity Relationship
Surface-Active Agents - pharmacology
Vertebrates: cardiovascular system
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Summary:This study examined the role of fatty acids on the phosphatidylcholine-specific phospholipase D (PLD) function of purified sarcolemmal (SL) membranes isolated from rat hearts. The enzyme's hydrolytic activity was determined by measuring [ 14C] phosphatidic acid formation from exogenous [ 14C] phosphatidylcholine (PtdCho) in the absence or presence of the sodium salts of various saturated or unsaturated long-chain fatty acids (FA). In certain experiments the enzyme was also assayed in the transphosphatidylation mode. Cisunsaturation and free carboxyl groups were structural prerequisites for the stimulatory effect exerted by FA on SL PLD. The most effective compounds were arachidonate and oleate, which maximally activated PLD at 4 and 5 mM concentration, respectively. To verify if a detergent-like mechanism was involved in PLD activation, anionic, zwitterionic and non-ionic detergents were used. Only anionic taurodeoxycholate had a slight effect, which was about 7% of that achieved by arachidonate or oleate. These results suggest that cis-unsaturated FA activate cardiac sarcolemmal PLD by a mechanism(s) which seems to be unrelated to non-specific perturbation of the membrane.
ISSN:0952-3278
1532-2823
DOI:10.1016/0952-3278(95)90017-9