Epitope Analysis Using Anti-Oligosaccharide (G4) Monoclonal Antibody

A murine monoclonal antibody recognizing (1→6)-β-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity wi...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(1), pp.43-47
Main Authors: Hirata, Akio, Komoda, Makiko, Itoh, Wataru, Tabata, Kengo, Sugawara, Isamu
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigenic determinants, haptens, artificial antigens
Antigens
Biological and medical sciences
Carbohydrate Sequence
Epitope
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glucans - immunology
Immunoglobulin M - immunology
Macrophages - immunology
Mice
Mice, Inbred BALB C
Molecular immunology
Molecular Sequence Data
Molecular Weight
Monoclonal antibody
Oligosaccharide
Oligosaccharides - immunology
Rabbits
Schizophyllan
Sizofiran - immunology
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Summary:A murine monoclonal antibody recognizing (1→6)-β-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyllan, an antitumor polysaccharide, but notable reactivity with some low-molecular-weight schizophyllans. This antibody was useful for determination of the epitope of several polysaccharides. The extent of reactivity of this monoclonal antibody was related only to the molecular weight of schizophyllan.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1995.tb02166.x