Chalcone synthase-like genes active during corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida (Asteraceae)

Recent studies on chalcone synthase (CHS) and the related stilbene synthase (STS) suggest that the structure of chs-like genes in plants has evolved into different forms, whose members have both different regulation and capacity to code for different but related enzymatic activities. We have studied...

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Bibliographic Details
Published in:Plant molecular biology 1995-04, Vol.28 (1), p.47-60
Main Authors: Helariutta, Y. (Helsinki Univ. (Finland). Inst. of Biotechnology), Elomaa, P, Kotilainen, M, Griesbach, R.J, Schroeder, J, Teeri, T.H
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - metabolism
Amino Acid Sequence
Base Sequence
CALCONA
CHALCONE
CHALCONES
CODE GENETIQUE
CODIGO GENETICO
ENZIMAS
ENZYME
ENZYMES
EXPRESION GENICA
EXPRESSION DES GENES
Flavonoids - analysis
Flavonoids - biosynthesis
Flavonols
FLORACION
FLORAISON
FLOWERING
GENE
GENE EXPRESSION
GENES
Genes, Plant - genetics
GENETIC CODE
GERBERA
Gerbera hybrida
In Situ Hybridization
Molecular Sequence Data
Plant Development
Plants - enzymology
Plants - genetics
RNA, Messenger - isolation & purification
Sequence Homology, Amino Acid
Substrate Specificity
Time Factors
Tissue Distribution
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Summary:Recent studies on chalcone synthase (CHS) and the related stilbene synthase (STS) suggest that the structure of chs-like genes in plants has evolved into different forms, whose members have both different regulation and capacity to code for different but related enzymatic activities. We have studied the diversity of chs-like genes by analysing the structure, expression patterns and catalytic properties of the corresponding enzymes of three genes that are active during corolla development in Gerbera hybrida. The expression patterns demonstrate that chs-like genes are representatives of three distinct genetic programmes that are active during organ differentiation in gerbera. Gchs1 and gchs3 code for typical CHS enzymes, and their gene expression pattern temporally correlates with flavonol (gchs1, gchs3) and anthocyanin (gchs1) synthesis during corolla development. Gchs2 is different. The expression pattern does not correlate with the pigmentation pattern, the amino acid sequence deviates considerably from the consensus of typical CHSs, and the catalytic properties are different. The data indicate that it represents a new member in the large superfamily of chs and chs-related genes.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00042037