Stimulatory factors for enzymatic biotin synthesis from dethiobiotin in cell-free extracts of Escherichia coli

The activity of biotin synthesis from dethiobiotin was found in cell-free extracts of an Escherichia coli bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine (AdoMet) had a significant effect, while methionine and cysteine were inert. The activity was linearly stimulat...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995-05, Vol.59 (5), p.943-944
Main Authors: OHSHIRO, T, YAMAMOTO, M, BUI, B. T. S, FLORENTIN, D, MARQUET, A, IZUMI, Y
Format: Article
Language:English
Subjects:
Bacillus - enzymology
Bacillus - metabolism
Bacteriology
Biological and medical sciences
Biotechnology
Biotin - analogs & derivatives
Biotin - biosynthesis
Biotin - metabolism
Cell Extracts - chemistry
Cysteine - pharmacology
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - metabolism
Flavin-Adenine Dinucleotide - metabolism
Flavin-Adenine Dinucleotide - pharmacology
Fundamental and applied biological sciences. Psychology
Immunosuppressive Agents
Metabolism. Enzymes
Microbiology
NAD - metabolism
NAD - pharmacology
NADP - metabolism
NADP - pharmacology
S-Adenosylmethionine - metabolism
S-Adenosylmethionine - pharmacology
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Summary:The activity of biotin synthesis from dethiobiotin was found in cell-free extracts of an Escherichia coli bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine (AdoMet) had a significant effect, while methionine and cysteine were inert. The activity was linearly stimulated by increasing protein concentration. When the dialyzed cell-free extracts were used for the reaction, NADP+, NADPH, and FAD among the well-known cofactors tested promoted the activity. Furthermore, in the presence of AdoMet, cysteine was apparently effective for biotin synthetic activity.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.943