Gene Expression and Phosphorylation of Mouse Osteopontin

Osteopontin is expressed in many different cell types and has been proposed to play several functions. Distinct forms of the protein have been detected. Various tissues and cell lines from mouse, however, exhibit two classes of transcripts with different 5'-untranslated ends but with an identic...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1995-08, Vol.760 (1), p.35-43
Main Authors: SAAVEDRA, RAUL A., KIMBRO, SEAN K., STERN, DORIS N., SCHNUER, JAMIE, ASHKAR, SAMY, GLIMCHER, MELVIN J., LJUBETIC, CECILIA I.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Adhesion
DNA Primers - chemistry
Gene Expression Regulation, Developmental
Mice
Molecular Sequence Data
Osteopontin
Phosphoproteins - metabolism
Phosphorylation
Phosphotyrosine
RNA, Messenger - genetics
Sialoglycoproteins - metabolism
Tyrosine - analogs & derivatives
Tyrosine - metabolism
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Summary:Osteopontin is expressed in many different cell types and has been proposed to play several functions. Distinct forms of the protein have been detected. Various tissues and cell lines from mouse, however, exhibit two classes of transcripts with different 5'-untranslated ends but with an identical coding region (exons II through VII). These transcripts do not arise from the alternative splicing of coding exons. These results suggest that posttranslational modifications of osteopontin, such as phosphorylation, are a major mechanism to generate different forms of the protein. Mouse osteopontin was expressed in E. coli and used as a model to study its phosphorylation.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1995.tb44618.x