additional class II intron with homology to reverse transcriptase in rapidly senescing Podospora anserina

Senescence in Podospora anserina is maternally inherited and the parameters of senescence are race specific. We have compared the restriction enzyme fragment maps of race A, the most rapidly senescing race, with race s and have found three inserts in race A which are not present in race s mitochondr...

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Bibliographic Details
Published in:Current genetics 1986-01, Vol.10 (12), p.915-922
Main Authors: Matsuura, E.T, Domenico, J.M, Cummings, D.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Ascomycota - enzymology
Ascomycota - genetics
Base Sequence
DNA
DNA, Fungal - genetics
enzymes
fungi
Genes, Fungal
genomics
Introns
mitochondria
Molecular Sequence Data
Nucleic Acid Conformation
Podospora anserina
RNA-Directed DNA Polymerase - genetics
senescence
Sequence Homology, Nucleic Acid
sequences
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Summary:Senescence in Podospora anserina is maternally inherited and the parameters of senescence are race specific. We have compared the restriction enzyme fragment maps of race A, the most rapidly senescing race, with race s and have found three inserts in race A which are not present in race s mitochondrial DNA. Fragment A was mapped and found to be located downstream of the so-called alpha senDNA, a class II intron, near the 5' end of the COI gene, separated from alpha senDNA by two class I introns. DNA sequence analysis showed that fragment A is also a class II intron, but with only 10% DNA sequence homology to alpha senDNA. Like alpha senDNA, intron A contains significant amino acid homology with known reverse transcriptases. The importance of this additional class II intron in the mitochondrial genome with the relative rate of senescence in race A is discussed.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00398289