new mutation for multiple drug resistance and modified plasma membrane ATPase activity in Schizosaccharomyces pombe

The mutant JV66 was selected from the wild type strain of S. pombe 972h- ade7-413 by its ability to grow on solid rich medium containing 200 micrograms Dio-9/ml. The single nuclear mutation, designated pma1 gives resistance towards diguanidines and several other positively charged compounds. The pma...

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Bibliographic Details
Published in:Current genetics 1986, Vol.10 (5), p.359-364
Main Authors: Ulaszewski, S, Coddington, A, Goffeau, A
Format: Article
Language:English
Subjects:
adenosinetriphosphatase
Biological and medical sciences
Biological Transport
Cell Membrane - enzymology
Cell physiology
Chromosome Mapping
drug resistance
Drug Resistance, Microbial
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Membrane and intracellular transports
Molecular and cellular biology
mutation
Nucleotidyltransferases - genetics
phenotype
plasma membrane
plasma membranes
resistant mutant
Saccharomycetales - genetics
Schizosaccharomyces - drug effects
Schizosaccharomyces - genetics
Schizosaccharomyces pombe
yeasts
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Description
Summary:The mutant JV66 was selected from the wild type strain of S. pombe 972h- ade7-413 by its ability to grow on solid rich medium containing 200 micrograms Dio-9/ml. The single nuclear mutation, designated pma1 gives resistance towards diguanidines and several other positively charged compounds. The pma1 mutation also decreases plasma membrane ATPase activity and confers resistance of ATPase to vanadate. The pma1 locus is localized on chromosome I at 5.3 map units from cyh1-C7 and at about 20.7 map units from the centromere. This new mutation is genetically and phenotypically different from the mutation cyh3 and cyh4 previously described (Johnston and Coddington 1983).
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00418407