Human vascular endothelial cells process and present autoantigen to human T cell lines

• Published in: International immunology 1995-03, Vol.7 (3), p.471-479
• Main Authors: Savage, Caroline O. S., Brooks, Christopher J., Harcourt, Gillian C., Picard, Jean K., King, William, Sansom, David M., Willcox, Nicholas
• Format: Article
• Language: English
• Subjects: Accessory cells; Amino acids; Antigen presentation; Antigen Presentation - drug effects; Antigens, CD - immunology; Autoantigens - immunology; B7-1 Antigen - physiology; Blood; CD28 antigen; CD28 Antigens - immunology; CD58 antigen; CD58 Antigens; Cell activation; Cell Adhesion Molecules - physiology; Cell lines; Cells, Cultured; Chloroquine; Chloroquine - pharmacology; Endothelial cells; Endothelium, Vascular - drug effects; Endothelium, Vascular - immunology; Epitopes; Epitopes - immunology; Histocompatibility antigen HLA; HLA-DQ Antigens - immunology; HLA-DR Antigens - immunology; Humans; Interferon-gamma - pharmacology; Lymphocytes; Lymphocytes T; Membrane Glycoproteins - immunology; Myasthenia; Myasthenia gravis; Myasthenia Gravis - immunology; Myasthenia Gravis - pathology; Neuromuscular junctions; Peptide Fragments - immunology; Peptides; Peripheral blood; Receptors; Receptors, Cholinergic - immunology; Signal Transduction; Signaling; T lymphocytes; T-Lymphocytes - immunology; Umbilical vein; Umbilical Veins; γ-Interferon
• ISSN: 0953-8178; 1460-2377
• DOI: 10.1093/intimm/7.3.471

The effectiveness of cultured human umbilical vein endothelial cells as accessory cells for T cell activation has been investigated using T cell clones and lines derived from patients with myasthenla gravis which were specific for different epitopes on the α subunit of the human acetylcholine receptor. The endothelial cells were induced with IFN-γ to express HLA-DR and -DQ at high and low levels respectively. They could then efficiently present specific peptides of the α subunit to an HLA-DR- and an HLA-DQw5-restricted T cell line. They could also process epitopes for both T cell lines from the full-length recombinant a subunlt (r1—437) of the human acetylcholine receptor, where the known epitopes are 80 amino acid residues apart. The endothelial presentation of r1—437, but not of the peptides, was sensitive to chloroquine inhibition. Presentation appeared slightly less efficient (by 1.5- to 3.0-fold) with endothelial cells than with presenting cells from peripheral blood. This may reflect differences in accessory signalling since mAb blocking studies suggested that ligands for CD28 provided important accessory signalling by peripheral blood presenting cells while LFA-3 was used by endothelial cells.