Gene expression of epimorphin in rat incisor ameloblasts

Epimorphin has been recently identified as an important factor in the morphogenesis of epithelial cells. A cDNA encoding epimorphin from skin of newborn mice was cloned by the polymerase chain-reaction technique before the preparation of digoxigenin-labelled cRNA probes. In situ hybridization of lon...

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Bibliographic Details
Published in:Archives of oral biology 1995-02, Vol.40 (2), p.161-164
Main Authors: Matsuki, Yutaka, Amizuka, Norio, Warshawsky, Hershey, Goltzman, David, Yamada, Yoshihiko
Format: Article
Language:English
Subjects:
ameloblasts
Ameloblasts - metabolism
Amelogenesis - genetics
Animals
Cell Differentiation - genetics
Cloning, Molecular
Dentistry
epimorphin
Epithelial Cells
Epithelium - metabolism
Gene Expression Regulation
In Situ Hybridization
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Mesoderm - metabolism
Mice
Molar
Morphogenesis - genetics
Polymerase Chain Reaction
rat incisor
Rats
Rats, Sprague-Dawley
RNA Probes
RNA, Complementary - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Skin
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Summary:Epimorphin has been recently identified as an important factor in the morphogenesis of epithelial cells. A cDNA encoding epimorphin from skin of newborn mice was cloned by the polymerase chain-reaction technique before the preparation of digoxigenin-labelled cRNA probes. In situ hybridization of longitudinal sections of rat incisors revealed a distinct pattern of expression of epimorphin mRNA in the ameloblast layer. Epimorphin mRNA was detected from the presecretory stage up to the beginning of the maturation stage of amelogenesis. With the identification of this expression by epithelial-derived cells, i.e. ameloblasts, it is thought likely that epimorphin is one of the factors that modulate the differentiation cascade of ameloblasts in the course of amelogenesis.
ISSN:0003-9969
1879-1506
DOI:10.1016/0003-9969(94)00150-A