Changes in the concentration of the calcium-binding parvalbumin in cross-reinnervated rat muscles. Comparison of biochemical with physiological and histochemical parameters

The fast extensor digitorum longus (EDL) and the slow soleus (SOL) muscles were cross-reinnervated in both directions in the rat. During the following transformation of muscle type properties, the expression of the Ca2+-binding parvalbumin (parvalbumin, Mr = 12,000) was investigated. The combined bi...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-01, Vol.262 (1), p.465-469
Main Authors: Müntener, M, Rowlerson, A M, Berchtold, M W, Heizmann, C W
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - metabolism
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Histocytochemistry
Immunoenzyme Techniques
Kinetics
Male
Muscle Contraction
Muscle Proteins - metabolism
Muscle Relaxation
Muscles - innervation
Muscles - metabolism
Parvalbumins - genetics
Parvalbumins - metabolism
Rats
RNA, Messenger - metabolism
Striated muscle. Tendons
Transcription, Genetic
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:The fast extensor digitorum longus (EDL) and the slow soleus (SOL) muscles were cross-reinnervated in both directions in the rat. During the following transformation of muscle type properties, the expression of the Ca2+-binding parvalbumin (parvalbumin, Mr = 12,000) was investigated. The combined biochemical, histochemical, and physiological results demonstrated that the amount of parvalbumin decreased in the fast to slow (X-EDL) and increased in the slow to fast (X-SOL) transformation. Alterations of parvalbumin-mRNA levels were similar to changes found at the protein level, indicating a tight transcriptional regulation of the parvalbumin expression. The close correlation, however, between parvalbumin and relaxation speed found in normal muscles had changed after cross-reinnervation. After the altered nervous input, a slow contracting/slow relaxing muscle may even contain more parvalbumin than a fast contracting/fast relaxing one. The expression of parvalbumin may depend on the nerve-muscle interaction, and parvalbumin may thus be used as a sensitive marker for early stages of muscular transformation and neurological disorders.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)75950-X