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Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis
Cerebrospinal fluid (CSF) and serum specimens from patients with presumed herpes simplex encephalitis (HSE) were characterized for antibodies to herpes simplex virus (HSV) by immunoblot and other immunoassays. Specimens from patients proven to have HSE (biopsy-proven) were compared with those from p...
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| Published in: | The Journal of infectious diseases 1987-01, Vol.155 (1), p.38-44 |
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| Main Authors: | , , , , , |
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| container_end_page | 44 |
| container_issue | 1 |
| container_start_page | 38 |
| container_title | The Journal of infectious diseases |
| container_volume | 155 |
| creator | Kahlon, Jasbir Chatterjee, Subhendra Lakeman, Fred D. Lee, Frances Nahmias, Andre J. Whitley, Richard J. |
| description | Cerebrospinal fluid (CSF) and serum specimens from patients with presumed herpes simplex encephalitis (HSE) were characterized for antibodies to herpes simplex virus (HSV) by immunoblot and other immunoassays. Specimens from patients proven to have HSE (biopsy-proven) were compared with those from patients with other diseases diagnosed by brain biopsy (biopsy-negative for HSV). Immunoblot of CSF demonstrated that antibodies to HSV-specific polypeptides, particularly to glycoprotein B, were present in a matched serum specimen. When purified glycoprotein B was used to detect CSF antibodies, 34 of 35 specimens from biopsy-proven patients were reactive (97% sensitivity) compared with only six of 22 specimens obtained from biopsy-negative patients (73% specificity). If leakage of antibodies to a marker virus (adenovirus) was determined and controlled, the specificity increased to 100%. These diagnostic assays provide useful tools for the retrospective assessment of CSF specimens obtained from patients with presumed HSE. |
| doi_str_mv | 10.1093/infdis/155.1.38 |
| format | article |
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Specimens from patients proven to have HSE (biopsy-proven) were compared with those from patients with other diseases diagnosed by brain biopsy (biopsy-negative for HSV). Immunoblot of CSF demonstrated that antibodies to HSV-specific polypeptides, particularly to glycoprotein B, were present in a matched serum specimen. When purified glycoprotein B was used to detect CSF antibodies, 34 of 35 specimens from biopsy-proven patients were reactive (97% sensitivity) compared with only six of 22 specimens obtained from biopsy-negative patients (73% specificity). If leakage of antibodies to a marker virus (adenovirus) was determined and controlled, the specificity increased to 100%. These diagnostic assays provide useful tools for the retrospective assessment of CSF specimens obtained from patients with presumed HSE.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/155.1.38</identifier><identifier>PMID: 3025307</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adenoviruses ; Adolescent ; Adult ; Aged ; Antibodies ; Antibodies, Viral - cerebrospinal fluid ; Antigens ; Biological and medical sciences ; Biopsies ; Biopsy ; Brain - pathology ; Cerebrospinal fluid ; Child ; Encephalitis ; Encephalitis - cerebrospinal fluid ; Encephalitis - immunology ; Encephalitis - pathology ; Herpes Simplex - cerebrospinal fluid ; Herpes Simplex - immunology ; Herpes Simplex - pathology ; herpes simplex virus ; Human viral diseases ; Humans ; Immunologic Techniques ; Infections ; Infectious diseases ; Medical sciences ; Middle Aged ; Original Articles ; Simplexvirus ; Simplexvirus - immunology ; Specimens ; Viral diseases ; Viral diseases of the nervous system ; Viral Envelope Proteins - immunology ; Viruses</subject><ispartof>The Journal of infectious diseases, 1987-01, Vol.155 (1), p.38-44</ispartof><rights>Copyright 1987 University of Chicago</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-edeec7a1ee4042b79b8d6a0835007919fe728f1019d41e03d3deee3367bba53e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30105718$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30105718$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,58213,58446</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8025620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3025307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kahlon, Jasbir</creatorcontrib><creatorcontrib>Chatterjee, Subhendra</creatorcontrib><creatorcontrib>Lakeman, Fred D.</creatorcontrib><creatorcontrib>Lee, Frances</creatorcontrib><creatorcontrib>Nahmias, Andre J.</creatorcontrib><creatorcontrib>Whitley, Richard J.</creatorcontrib><title>Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Cerebrospinal fluid (CSF) and serum specimens from patients with presumed herpes simplex encephalitis (HSE) were characterized for antibodies to herpes simplex virus (HSV) by immunoblot and other immunoassays. Specimens from patients proven to have HSE (biopsy-proven) were compared with those from patients with other diseases diagnosed by brain biopsy (biopsy-negative for HSV). Immunoblot of CSF demonstrated that antibodies to HSV-specific polypeptides, particularly to glycoprotein B, were present in a matched serum specimen. When purified glycoprotein B was used to detect CSF antibodies, 34 of 35 specimens from biopsy-proven patients were reactive (97% sensitivity) compared with only six of 22 specimens obtained from biopsy-negative patients (73% specificity). If leakage of antibodies to a marker virus (adenovirus) was determined and controlled, the specificity increased to 100%. These diagnostic assays provide useful tools for the retrospective assessment of CSF specimens obtained from patients with presumed HSE.</description><subject>Adenoviruses</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibodies</subject><subject>Antibodies, Viral - cerebrospinal fluid</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Biopsies</subject><subject>Biopsy</subject><subject>Brain - pathology</subject><subject>Cerebrospinal fluid</subject><subject>Child</subject><subject>Encephalitis</subject><subject>Encephalitis - cerebrospinal fluid</subject><subject>Encephalitis - immunology</subject><subject>Encephalitis - pathology</subject><subject>Herpes Simplex - cerebrospinal fluid</subject><subject>Herpes Simplex - immunology</subject><subject>Herpes Simplex - pathology</subject><subject>herpes simplex virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunologic Techniques</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Original Articles</subject><subject>Simplexvirus</subject><subject>Simplexvirus - immunology</subject><subject>Specimens</subject><subject>Viral diseases</subject><subject>Viral diseases of the nervous system</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqFkUtr3DAURkVpSCdp110VtCjdeebKetnLMHlMIaFT-iB0I2T7mlHqsV1JJsm_j4aZpqWbrnThO_eIy0fIWwZzBiVfuL5tXFgwKedszosXZMYk15lSjL8kM4A8z1hRlq_ISQh3ACC40sfkmEMuOegZuT_HiHV0Q0-Hlp710VVD4zDQONAV-jFNX9x27PCBfnd-CtT1NG6QLtFj5Ycwut529LKbXLMTrG102MdA713c_Cu46GscN7Zz0YXX5Ki1XcA3h_eUfLu8-LpcZdefrj4uz66zWrA8Ztgg1toyRAEir3RZFY2yUHAJoEtWtqjzomXAykYwBN7wtIA8HVlVVnLkp-TD3jv64deEIZqtCzV2ne1xmILRmishQP0XZEKDUIVM4GIP1un84LE1o3db6x8NA7PrxOw7MakTwwwv0sa7g3qqttg884cSUv7-kNtQ2671tq_T_m-sSJjK4Y_mLsTB_2VhIDXbfZPtcxciPjzn1v80SnMtzer2h7lZn-tbuV6az_wJ64yvuQ</recordid><startdate>198701</startdate><enddate>198701</enddate><creator>Kahlon, Jasbir</creator><creator>Chatterjee, Subhendra</creator><creator>Lakeman, Fred D.</creator><creator>Lee, Frances</creator><creator>Nahmias, Andre J.</creator><creator>Whitley, Richard J.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198701</creationdate><title>Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis</title><author>Kahlon, Jasbir ; Chatterjee, Subhendra ; Lakeman, Fred D. ; Lee, Frances ; Nahmias, Andre J. ; Whitley, Richard J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-edeec7a1ee4042b79b8d6a0835007919fe728f1019d41e03d3deee3367bba53e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adenoviruses</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Antibodies</topic><topic>Antibodies, Viral - cerebrospinal fluid</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Biopsies</topic><topic>Biopsy</topic><topic>Brain - pathology</topic><topic>Cerebrospinal fluid</topic><topic>Child</topic><topic>Encephalitis</topic><topic>Encephalitis - cerebrospinal fluid</topic><topic>Encephalitis - immunology</topic><topic>Encephalitis - pathology</topic><topic>Herpes Simplex - cerebrospinal fluid</topic><topic>Herpes Simplex - immunology</topic><topic>Herpes Simplex - pathology</topic><topic>herpes simplex virus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunologic Techniques</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Original Articles</topic><topic>Simplexvirus</topic><topic>Simplexvirus - immunology</topic><topic>Specimens</topic><topic>Viral diseases</topic><topic>Viral diseases of the nervous system</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kahlon, Jasbir</creatorcontrib><creatorcontrib>Chatterjee, Subhendra</creatorcontrib><creatorcontrib>Lakeman, Fred D.</creatorcontrib><creatorcontrib>Lee, Frances</creatorcontrib><creatorcontrib>Nahmias, Andre J.</creatorcontrib><creatorcontrib>Whitley, Richard J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kahlon, Jasbir</au><au>Chatterjee, Subhendra</au><au>Lakeman, Fred D.</au><au>Lee, Frances</au><au>Nahmias, Andre J.</au><au>Whitley, Richard J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1987-01</date><risdate>1987</risdate><volume>155</volume><issue>1</issue><spage>38</spage><epage>44</epage><pages>38-44</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Cerebrospinal fluid (CSF) and serum specimens from patients with presumed herpes simplex encephalitis (HSE) were characterized for antibodies to herpes simplex virus (HSV) by immunoblot and other immunoassays. Specimens from patients proven to have HSE (biopsy-proven) were compared with those from patients with other diseases diagnosed by brain biopsy (biopsy-negative for HSV). Immunoblot of CSF demonstrated that antibodies to HSV-specific polypeptides, particularly to glycoprotein B, were present in a matched serum specimen. When purified glycoprotein B was used to detect CSF antibodies, 34 of 35 specimens from biopsy-proven patients were reactive (97% sensitivity) compared with only six of 22 specimens obtained from biopsy-negative patients (73% specificity). If leakage of antibodies to a marker virus (adenovirus) was determined and controlled, the specificity increased to 100%. These diagnostic assays provide useful tools for the retrospective assessment of CSF specimens obtained from patients with presumed HSE.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>3025307</pmid><doi>10.1093/infdis/155.1.38</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1987-01, Vol.155 (1), p.38-44 |
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| source | JSTOR Archival Journals and Primary Sources Collection; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025 |
| subjects | Adenoviruses Adolescent Adult Aged Antibodies Antibodies, Viral - cerebrospinal fluid Antigens Biological and medical sciences Biopsies Biopsy Brain - pathology Cerebrospinal fluid Child Encephalitis Encephalitis - cerebrospinal fluid Encephalitis - immunology Encephalitis - pathology Herpes Simplex - cerebrospinal fluid Herpes Simplex - immunology Herpes Simplex - pathology herpes simplex virus Human viral diseases Humans Immunologic Techniques Infections Infectious diseases Medical sciences Middle Aged Original Articles Simplexvirus Simplexvirus - immunology Specimens Viral diseases Viral diseases of the nervous system Viral Envelope Proteins - immunology Viruses |
| title | Detection of Antibodies to Herpes Simplex Virus in the Cerebrospinal Fluid of Patients with Herpes Simplex Encephalitis |
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