Sequence analysis of canine p53 in the region of exons 3–8

An 828 base pair region of canine wild-type p53, corresponding to codons 25 through 312 in the human p53 gene, was directly sequenced from asymmetric polymerase chain reaction (PCR) products. The deduced amino acid sequence of the dog was 86%, 73% and 83% homologous to that of the cat, mouse and hum...

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Bibliographic Details
Published in:Cancer letters 1995-06, Vol.92 (2), p.181-186
Main Authors: Kraegel, Susan A., Pazzi, Karen A., Madewell, Bruce R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cats
Classical genetics, quantitative genetics, hybrids
Direct sequencing
DNA - genetics
DNA Primers
Dog
Dogs
Exons
Fundamental and applied biological sciences. Psychology
Gene Amplification
Genes, p53
Genetics of eukaryotes. Biological and molecular evolution
Humans
Mice
Molecular Sequence Data
p53
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Species Specificity
Tumor Suppressor Protein p53 - genetics
Vertebrata
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Summary:An 828 base pair region of canine wild-type p53, corresponding to codons 25 through 312 in the human p53 gene, was directly sequenced from asymmetric polymerase chain reaction (PCR) products. The deduced amino acid sequence of the dog was 86%, 73% and 83% homologous to that of the cat, mouse and human proteins, respectively. In the evolutionarily conserved domains II through V, the canine sequence approached 100% homology with the human sequence. The region sequenced encompasses exons 3–8 where the majority of mutations have been identified in neoplastic specimens from human patients.
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(95)03779-V