Decreased uptake of taurocholate and ouabain by hepatocytes isolated from cirrhotic rat liver

To differentiate between the “intact” and “sick” cell hypothesis explaining decreased clearance of endo‐ and xenobiotics, we measured uptake of taurocholate and ouabain in hepatocytes isolated from cirrhotic rat liver. Cirrhosis was induced by chronic exposure of male Sprague‐Dawley rats to phenobar...

Full description

Saved in:
Bibliographic Details
Published in:Hepatology (Baltimore, Md.) Md.), 1987-01, Vol.7 (1), p.67-70
Main Authors: Reichen, Jürg, Hoilien, Catherine, Le, Mysan, Jones, Richard H.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
General aspects. Methods
Liver - cytology
Liver - metabolism
Liver Cirrhosis, Experimental - metabolism
Male
Medical sciences
Ouabain - metabolism
Oxygen Consumption
Rats
Rats, Inbred Strains
Taurocholic Acid - metabolism
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To differentiate between the “intact” and “sick” cell hypothesis explaining decreased clearance of endo‐ and xenobiotics, we measured uptake of taurocholate and ouabain in hepatocytes isolated from cirrhotic rat liver. Cirrhosis was induced by chronic exposure of male Sprague‐Dawley rats to phenobarbital and carbon tetrachloride. Uptake of [14C]taurocholate and [3H]ouabain was measured by a rapid filtration technique. Hepatocytes from cirrhotic liver were as viable as control hepatocytes—as judged by trypan blue exclusion and lactate dehydrogenase release—but consumed 28% less oxygen. Vmax of both taurocholate (3.16 ± 0.95 vs. 0.40 ± 0.35 nmoles·min−1·106 cells−1; p < 0.001) and ouabain (2.16 ± 0.78 vs. 0.83 ± 0.26 nmoles·min−1·106 cells−1; p < 0.005) was significantly reduced. These results are compatible with the “sick” cell hypothesis.
ISSN:0270-9139
1527-3350
DOI:10.1002/hep.1840070115