Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC

• Published in: Immunity (Cambridge, Mass.) Mass.), 1995, Vol.2 (1), p.113-123
• Main Authors: Girard, Jean-Philippe, Springer, Timothy A.
• Format: Article
• Language: English
• Subjects: Activated-Leukocyte Cell Adhesion Molecule; Amino Acid Sequence; Animals; Antibodies, Monoclonal - immunology; Base Sequence; calcium-binding glycoprotein; Calcium-Binding Proteins - chemistry; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - immunology; cDNA; Cell Adhesion; Cloning, Molecular; Coturnix; DNA, Complementary - genetics; endothelium; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; extracellular matrix; Extracellular Matrix Proteins - chemistry; Eye Proteins - chemistry; Fluorescent Antibody Technique; Gene Library; Genes; Glycoproteins - chemistry; Glycoproteins - genetics; Glycoproteins - immunology; hevin; Humans; Immunomagnetic Separation; In Situ Hybridization; man; Molecular Sequence Data; Multigene Family; Nerve Tissue Proteins - chemistry; nucleotide sequence; Osteonectin - chemistry; Palatine Tonsil - cytology; prediction; proteins; Rats; RNA, Messenger - genetics; Sequence Alignment; Sequence Homology, Amino Acid; similarity; tonsil; Umbilical Veins
• ISSN: 1074-7613; 1097-4180
• DOI: 10.1016/1074-7613(95)90083-7

High endothelial venules (HEV) in lymphoid tissues support high levels of lymphocyte extravasion from the blood. We purified high endothelial cells from human tonsils by immunomagnetic selection with MECA79 MAb to construct an HEV cDNA.library. Differential screening of this library using cDNA probes from HEV (plus) or flat-walled vessel (minus) endothelial cells allowed us to characterize a novel human cDNA expressed to high levels in HEV. The cDNA encodes a secreted acidic calcium-binding glycoprotein of 664 as residues, designated hevin, exhibiting 62% identity with the antiadhesive extracellular matrix protein SPARC, over a region of 232 as spanning more than four fifths of the SPARC coding sequence. The primary structure and sequence of hevin are similar to SPARC41ke proteins from rat and quail, called SC1 or GR1. Hevin could contribute to the Induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.