A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its ‘death domain’

• Published in: FEBS letters 1995-06, Vol.367 (1), p.39-44
• Main Authors: Boldin, Mark P., Mett, Igor L., Wallach, David
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antigens, CD - metabolism; Apoptosis - genetics; Cloning, Molecular; Death domain; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Fas-IC, intracellular domain of Fas/APO1; FLAG-55.11, the region extending between residues 309 and 900 in the 55.11 protein and which was N-linked to the FLAG octapeptide; Gene Deletion; GST, glutathione S-transferase; HeLa Cells; Humans; IC, intracellular domain; Molecular Sequence Data; p55-IC, intracellular domain of the p55-R; p55-R, the p55 receptor for TNF; p75-R, the p75 receptor for TNF; Proteasome; Proteins - genetics; Proteins - isolation & purification; Proteins - metabolism; Receptor; Receptors, Tumor Necrosis Factor - metabolism; Receptors, Tumor Necrosis Factor, Type I; Sequence Alignment; Signaling; TNF Receptor-Associated Factor 2; TNF, tumor necrosis factor; Tumor necrosis factor; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; Two-hybrid system
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)00534-G

A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet unknown. Significant similarity was also found between 55.11 and SEN3, the yeast equivalent of the p112 subunit of the 26S proteasome. Deletion analysis showed that the protein binds to the p55 receptor upstream to the region involved in induction of cell death.