Hematologic Engraftment and Immune Reconstitution Posttransplantation With Anti-B1 Purged Autologous Bone Marrow

• Published in: Blood 1987-02, Vol.69 (2), p.597-604
• Main Authors: Anderson, K.C., Ritz, J., Takvorian, T., Coral, F., Daley, H., Gorgone, B.C., Freedman, A.S., Canellos, George P., Schlossman, S.F., Nadler, L.M.
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antibodies, Monoclonal - therapeutic use; Antigens, Neoplasm - immunology; Biological and medical sciences; Bone Marrow - immunology; Bone Marrow - pathology; Bone Marrow Transplantation; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Separation - methods; Graft Survival; Humans; Immunity, Cellular; Lymphoma, Non-Hodgkin - therapy; Medical sciences; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation, Autologous
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V69.2.597.597

Hematologic engraftment and immune reconstitution were examined in patients who received cyclophosphamide and total body irradiation therapy followed by infusion of autologous bone marrow purged with anti-B1 monoclonal antibody (MoAb) and complement as therapy for non-Hodg-kin' 's lymphoma. Hematologic engraftment was prompt with return of ≥ 0.5 × 103/µl granulocytes and ≥ 2 × 104/µL platelets at a median of 26 and 29 days posttrans-lant, respectively. Immunologic reconstitution, in contrast, was prolonged. Normal numbers of circulating B cells were consistently noted by five months posttransplant, whereas return of normal immunoglobulin levels in some patients did not occur for one year. Normal numbers of T cells were evident within the first month posttransplant, but a reversed T4:T8 ratio persisted in some patients up to three years. In vitro responses of either B cells to triggers of activation or of T cells to mitogens and antigens were not normal for at least three months posttransplant. Natural killer (NK) cells predominated early after transplant and may demonstrate cytotoxicity against tumor cells. Our studies demonstrate that transplantation with anti-B1 purged autologous bone marrow results in complete hematologic and delayed immunologic engraftment. No significant acute or chronic clinical toxicities have been observed. © 1987 by Grune & Stratton, Inc.