Effects of adenosine receptor agonists on nitric oxide release in mouse during endotoxemia

• Published in: Biochemical pharmacology 1995-06, Vol.50 (1), p.45-47
• Main Authors: Hon, Wei Min, Khoo, Hoon Eng, Ngoi, Sing Shang, Moochhala, Shabbir
• Format: Article
• Language: English
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; adenosine agonist; Adenosine-5'-(N-ethylcarboxamide); Amino Acid Oxidoreductases - biosynthesis; Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; endotoxemia; Enzyme Induction; lipopolysaccharide; Lipopolysaccharides - pharmacology; Male; Medical sciences; Mice; Miscellaneous. Antibiotics with multiple activities; nitric oxide; Nitric Oxide - biosynthesis; Nitric Oxide - blood; Nitric Oxide Synthase; Pharmacology. Drug treatments; Phenylisopropyladenosine - pharmacology; Purinergic P1 Receptor Agonists; Shock, Septic - blood
• ISSN: 0006-2952; 1873-2968
• DOI: 10.1016/0006-2952(95)00111-C

The effects of adenosine receptor agonists on plasma NO x − (NO 2 − and NO 3 −) production in mice treated with lipopolysaccharide (LPS) were investigated. NO x − is the stable decomposition product of nitric oxide (NO), which can be measured as a marker of NO production. Injection of the mice with LPS resulted in increased plasma NO x − concentration, reaching a peak after 8 hr (38 times basal level) and then declining slowly. Pretreatment with the adenosine agonists R-phenylisopropyladenosine ( R-PIA), 5′- N-ethylcarboxamidoadenosine (NECA), 5′-( N-cyclopropyl)carboxamidoadenosine (CPCA) and N 6-cyclohexyladenosine (CHA) 1 hr before LPS administration caused a dose-dependent reduction of plasma NO x −1 concentration. The rank order of inhibitory potency was NECA ≥ R-PIA CPCA CHA, which is characteristic of neither A 1 nor A 2 receptors.