Dengue 4 Virus Monoclonal Antibodies Identify Epitopes that Mediate Immune Infection Enhancement of Dengue 2 Viruses

1 Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, 3675 Kilauea Avenue, Honolulu, Hawaii 96816 2 Harvard Medical School, Boston, Massachusetts 3 The Rockefeller Foundation, New York, New York, U.S.A. Nineteen monoclonal antibodies pro...

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Bibliographic Details
Published in:Journal of general virology 1987-01, Vol.68 (1), p.91-98
Main Authors: Morens, David M, Venkateshan, C. N, Halstead, Scott B
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigen-Antibody Complex
Antigens, Viral - immunology
Arboviroses
Biological and medical sciences
Cell Line
Dengue - immunology
Dengue fevers
Dengue Virus - genetics
Dengue Virus - immunology
dengue virus 2
dengue virus 4
Epitopes - analysis
Fundamental and applied biological sciences. Psychology
Human viral diseases
Immunoassay
Infectious diseases
Medical sciences
Mice
Microbiology
Serotyping
Tropical medicine
Tropical viral diseases
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Viral diseases
Virology
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Summary:1 Department of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, 3675 Kilauea Avenue, Honolulu, Hawaii 96816 2 Harvard Medical School, Boston, Massachusetts 3 The Rockefeller Foundation, New York, New York, U.S.A. Nineteen monoclonal antibodies produced to dengue type 4 virus (DEN-4) strain 4328-S were tested for their ability to mediate antibody-dependent infection enhancement (ADE) with seven DEN-2 strains in P-388D1 mouse macrophage-like cells. In this first study of the distribution of enhancing epitopes on multiple DEN-2 strains reacted with monoclonal antibodies to a different serotype (DEN-4), DEN-4 monospecific antibodies produced ADE with DEN-2 viruses, indicating the presence of DEN-4-like determinants on DEN-2 viruses. Analysis differentiated at least one and possibly more DEN-2 strain subgroups, one of which (isolates AHF-110 and AHF-191) was previously identified by DEN-2 monoclonal antibody analysis. The study demonstrates the heterogeneous distribution of dengue complex and DEN-4 epitopes on DEN-2 strains. Monoclonal antibodies are valuable tools for study of the biology of ADE and its relation to dengue shock syndrome. Received 23 May 1986; accepted 9 September 1986.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-68-1-91